Gene expression changes in conjunctival cells associated with contact lens wear and discomfort

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface Pub Date : 2024-01-01 DOI:10.1016/j.jtos.2023.12.004

Andrés Ángel Calderón-García , Laura Valencia-Nieto , Cristina Valencia-Sandonis , Alberto López-de la Rosa , Marta Blanco-Vazquez , Itziar Fernández , Carmen García-Vázquez , Cristina Arroyo-del Arroyo , María J. González-García , Amalia Enríquez-de-Salamanca

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引用次数: 0

Abstract

Purpose

This study aimed to analyze the differences in the expression of pain-related genes in conjunctival epithelial cells among symptomatic contact lens (CL) wearers (SCLWs), asymptomatic CL wearers (ACLWs), and non-CL wearers (non-CLWs).

Methods

For this study, 60 participants (20 non-CLWs, 40 CLWs) were enrolled. The CLW group comprised 20 ACLWs and 20 SCLWs according to the Contact Lens Dry Eye Questionnaire short form©. Conjunctival cells were collected using impression cytology, and RNA was isolated and used to determine the expression levels of 85 human genes involved in neuropathic and inflammatory pain. The effects of CL wear and discomfort were evaluated using mixed-effects ANOVA with partially nested fixed-effects model. Gene set enrichment analysis was performed to assign biological meaning to sets of differentially expressed genes.

Results

Six genes (CD200, EDN1, GRIN1, PTGS1, P2RX7, and TNF) were significantly upregulated in CLWs compared to non-CLWs. Eleven genes (ADORA1, BDKRB1, CACNA1B, DBH, GRIN1, GRM1, HTR1A, PDYN, PTGS1, P2RX3, and TNF) were downregulated in SCLWs compared to ACLWs. These genes were mainly related to pain, synaptic transmission and signaling, ion transport, calcium transport and concentration, and cell-cell signaling.

Conclusions

CL wear modified the expression of pain- and inflammation-related genes in conjunctival epithelial cells. These changes may be in part, along with other mechanisms, responsible for CL discomfort in SCLWs.

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与隐形眼镜佩戴和不适有关的结膜细胞基因表达变化。

目的：本研究旨在分析有症状隐形眼镜佩戴者（SCLWs）、无症状隐形眼镜佩戴者（ACLWs）和非隐形眼镜佩戴者（non-CLWs）结膜上皮细胞中疼痛相关基因表达的差异：本研究共招募了 60 名参与者（20 名非 CLW，40 名 CLW）。根据隐形眼镜干眼症问卷简表，隐形眼镜干眼症组包括 20 名 ACLWs 和 20 名 SCLWs。采用印模细胞学方法收集结膜细胞，并分离 RNA，用于测定涉及神经性和炎症性疼痛的 85 个人类基因的表达水平。采用混合效应方差分析和部分嵌套固定效应模型评估了CL磨损和不适的影响。进行了基因组富集分析，以确定差异表达基因组的生物学意义：结果：CLWs 中有六个基因（CD200、EDN1、GRIN1、PTGS1、P2RX7 和 TNF）比非 CLWs 中的基因明显上调。与 ACLW 相比，11 个基因（ADORA1、BDKRB1、CACNA1B、DBH、GRIN1、GRM1、HTR1A、PDYN、PTGS1、P2RX3 和 TNF）在 SCLW 中下调。这些基因主要与疼痛、突触传递和信号传导、离子转运、钙转运和浓度以及细胞-细胞信号传导有关：结论：CL磨损改变了结膜上皮细胞中疼痛和炎症相关基因的表达。这些变化和其他机制可能是造成SCLW佩戴CL后不适的部分原因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ocular Surface 医学-眼科学

CiteScore

11.60

自引率

14.10%

发文量

审稿时长

39 days

期刊介绍： The Ocular Surface, a quarterly, a peer-reviewed journal, is an authoritative resource that integrates and interprets major findings in diverse fields related to the ocular surface, including ophthalmology, optometry, genetics, molecular biology, pharmacology, immunology, infectious disease, and epidemiology. Its critical review articles cover the most current knowledge on medical and surgical management of ocular surface pathology, new understandings of ocular surface physiology, the meaning of recent discoveries on how the ocular surface responds to injury and disease, and updates on drug and device development. The journal also publishes select original research reports and articles describing cutting-edge techniques and technology in the field. Benefits to authors We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services. Please see our Guide for Authors for information on article submission. If you require any further information or help, please visit our Support Center