Hydroalcoholic extract of Scrophularia striata has a significant therapeutic effect on thioacetamide-induced liver cirrhosis in rats.

IF 1.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Hormone Molecular Biology and Clinical Investigation Pub Date : 2023-12-22 eCollection Date: 2023-12-01 DOI:10.1515/hmbci-2022-0106

Jamal Amri, Mohammad Jamalian, Zahre Salami, Ahmad Akbari, Mona Alaee, Fatemeh Azizi Soleiman, Abbas Alimoradian

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引用次数: 0

Abstract

Objectives: Liver cirrhosis is one of the most important causes of death from liver diseases. Nowadays, the use of herbal medicines has increased due to its availability, less side effects and cheapness for the treatment of liver diseases. The present study was conducted to examine therapeutic effects of hydroalcoholic extract of Scrophularia striata (S. striata) on thioacetamide-induced liver cirrhosis in rats through evaluate its effects on oxidative stress markers and the expression of metalloproteinase 1 (TIMP 1), toll-like receptor-4 (TLR-4), and Mitofusin (MFN2) genes.

Methods: 24 male rats were selected by simple random sampling. Rats were randomly assigned to four groups: group I: healthy rats, group II: thioacetamide (TAA) injected rats, group III: TAA injected rats+100 mg/kg bw of S. striata and group IV: TAA injected rats+200 mg/kg bw of S. striata. Liver cirrhosis was induced in rats by a 300 mg/kg bw TAA administration twice with an interval of 24 h. After 8 weeks of treatment by S. striata at doses of 100 and 200 mg/kg bw, biochemical factors and oxidative stress markers (SOD, TAC, GPX, CAT and MDA) were measured using spectrophotometric methods. Also, gene expression of TIMP 1, TLR-4, and MFN2 were analyzed using real-time PCR. ANOVA and Bonferroni post hoc test analysis were applied to evaluate the data.

Results: The results showed the S. striata extract significantly improve the serum ALT, AST and ALP levels, TIMP 1, TLR-4, and MFN2 genes and oxidative stress markers (SOD, TAC, GPX, CAT and MDA) in the liver tissues when compared to control group (p<0.05). Also, it was found that the beneficial effects of the S. striata were dose-dependent.

Conclusions: Based on the results obtained S. striata by reducing the expression of TIMP 1, TLR-4, and MFN2 genes and improving oxidative stress might be used as adjuvant treatment for liver cirrhosis.

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条叶莨菪的水醇提取物对硫代乙酰胺诱导的大鼠肝硬化有显著的治疗作用。

目的：肝硬化是肝病致死的最主要原因之一。如今，草药因其易得、副作用小、价格便宜等特点，在肝病治疗中的使用越来越多。本研究通过评估黄芩水醇提取物对氧化应激标志物以及金属蛋白酶 1 (TIMP 1)、toll 样受体-4 (TLR-4) 和 Mitofusin (MFN2) 基因表达的影响，研究其对硫代乙酰胺诱导的大鼠肝硬化的治疗效果。大鼠随机分为四组：Ⅰ组：健康大鼠；Ⅱ组：注射硫代乙酰胺（TAA）大鼠；Ⅲ组：注射TAA+100 mg/kg体重的条纹叶藻大鼠；Ⅳ组：注射TAA+200 mg/kg体重的条纹叶藻大鼠。给大鼠注射两次 300 毫克/千克体重的 TAA，每次间隔 24 小时，诱发肝硬化。以 100 和 200 毫克/千克体重的剂量给大鼠注射横纹肌苷 8 周后，采用分光光度法测量生化因子和氧化应激标记物（SOD、TAC、GPX、CAT 和 MDA）。此外，还使用实时 PCR 分析了 TIMP 1、TLR-4 和 MFN2 的基因表达。对数据进行方差分析和 Bonferroni 后验分析：结果表明，与对照组相比，S. striata 提取物能明显改善血清 ALT、AST 和 ALP 水平、TIMP 1、TLR-4 和 MFN2 基因以及肝组织中的氧化应激标记物（SOD、TAC、GPX、CAT 和 MDA）：根据研究结果，S. striata 能降低 TIMP 1、TLR-4 和 MFN2 基因的表达，改善氧化应激，可作为肝硬化的辅助治疗药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hormone Molecular Biology and Clinical Investigation BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.60

自引率

0.00%

发文量

期刊介绍： Hormone Molecular Biology and Clinical Investigation (HMBCI) is dedicated to the provision of basic data on molecular aspects of hormones in physiology and pathophysiology. The journal covers the treatment of major diseases, such as endocrine cancers (breast, prostate, endometrium, ovary), renal and lymphoid carcinoma, hypertension, cardiovascular systems, osteoporosis, hormone deficiency in menopause and andropause, obesity, diabetes, brain and related diseases, metabolic syndrome, sexual dysfunction, fetal and pregnancy diseases, as well as the treatment of dysfunctions and deficiencies. HMBCI covers new data on the different steps and factors involved in the mechanism of hormone action. It will equally examine the relation of hormones with the immune system and its environment, as well as new developments in hormone measurements. HMBCI is a blind peer reviewed journal and publishes in English: Original articles, Reviews, Mini Reviews, Short Communications, Case Reports, Letters to the Editor and Opinion papers. Ahead-of-print publishing ensures faster processing of fully proof-read, DOI-citable articles.