Expression and functional characterization of bovine receptor activator of NF-κB ligand (RANKL)

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2023-12-21 DOI:10.1016/j.vetimm.2023.110705

Ambre F. Chapuis , Omar A. Alfituri , Jayne C. Hope , Jo Stevens , Jo Moore , Kevin Mclean , Dorota Androscuk , Inga Dry

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引用次数: 0

Abstract

Receptor activator of nuclear factor Kappa-B Ligand (RANKL) is a member of the tumor necrosis factor ligand (TNF) family involved in immune responses and immunomodulation. Expressed in various cells types around the body, RANKL plays a crucial role in bone remodeling and development of the thymus, lymph nodes and mammary glands. Research in other species demonstrates that RANKL is required for the development of microfold cells (M cells) in the gut, however limited information specific to cattle is available. Cloning and expression of bovine RANKL (BoRANKL) was carried out and bioactivity of the protein was demonstrated in the induction of osteoclast differentiation from both bovine and ovine bone marrow cells. The effects of BoRANKL on particle uptake in bovine enteroids was also assessed. The production of cross-reactive bovine RANKL protein will enable further investigations into cell differentiation using the available ruminant organoid systems, and their role in investigating host-pathogen interactions in cattle and sheep.

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牛 NF-κB 受体激活剂配体（RANKL）的表达和功能特性分析

核因子卡巴-B 受体激活剂配体（RANKL）是肿瘤坏死因子配体（TNF）家族的成员，参与免疫反应和免疫调节。RANKL 在人体的各种细胞中都有表达，在骨骼重塑以及胸腺、淋巴结和乳腺的发育过程中起着至关重要的作用。对其他物种的研究表明，RANKL 是肠道微褶细胞（M 细胞）发育所必需的，但有关牛的具体信息却很有限。对牛 RANKL（BoRANKL）进行了克隆和表达，并证明了该蛋白在诱导牛和绵羊骨髓细胞破骨细胞分化方面的生物活性。此外，还评估了 BoRANKL 对牛肠道微粒吸收的影响。交叉反应牛 RANKL 蛋白的生产将使人们能够利用现有的反刍动物类器官系统进一步研究细胞分化及其在研究牛羊宿主与病原体相互作用中的作用。

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.