Observation of non-equilibrium fluctuation in the shear-stress-driven hemoglobin aggregates

IF 1.8 4区物理与天体物理 Q4 CHEMISTRY, PHYSICAL

The European Physical Journal E Pub Date : 2023-12-20 DOI:10.1140/epje/s10189-023-00389-1

A. Kabiraj, G. Mallik, P. P. Dash, P. Kumari, M. Bandyopadhyay, S. Rath

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Abstract

Non-equilibrium fluctuations caused by the rearrangement of hemoglobin molecules into an aggregate state under shear stress have been investigated experimentally. The flow response under the shear stress (σ) corroborates the presence of contrasting aggregate and rejuvenation states governed by entropy production and consumption events. From the time-dependent shear rate fluctuation studies of aggregate states, the probability distribution function (PDF) of the rate of work done is observed to be spread from negative to positive values with a net positive mean. The PDFs follow the steady-state fluctuation theorem, even at a smaller timescale than that desired by the theorem. The behavior of the effective temperature (T_eff) that emerges from a non-equilibrium fluctuation and interconnects with the structural restrictions of the aggregate state of our driven system is observed to be within the boundary of the thermodynamic uncertainty. The increase in T_eff with the applied σ illustrates a phenomenal nonlinear power flux-dependent aggregating behavior in a classic bio-molecular-driven system.

Graphical abstract

Abstract Image

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在剪切应力驱动的血红蛋白聚集体中观察到非平衡波动

实验研究了血红蛋白分子在剪切应力作用下重新排列成聚集状态所引起的非平衡波动。剪切应力（σ）下的流动响应证实了在熵产生和消耗事件的支配下，存在着截然不同的聚集和恢复状态。通过对聚集状态的随时间变化的剪切速率波动研究，可以观察到做功速率的概率分布函数（PDF）从负值到正值分布，平均值为净正值。PDF 遵循稳态波动定理，即使时间尺度小于该定理所期望的时间尺度。从非平衡波动中产生的有效温度（Teff）与我们所驱动的系统总体状态的结构限制相互关联，其行为被观察到在热力学不确定性的边界之内。Teff 随应用 σ 的增加而增加，这说明在一个典型的生物分子驱动系统中，存在着惊人的非线性功率通量聚集行为。

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来源期刊

The European Physical Journal E CHEMISTRY, PHYSICAL-MATERIALS SCIENCE, MULTIDISCIPLINARY

CiteScore

2.60

自引率

5.60%

发文量

审稿时长

3 months

期刊介绍： EPJ E publishes papers describing advances in the understanding of physical aspects of Soft, Liquid and Living Systems. Soft matter is a generic term for a large group of condensed, often heterogeneous systems -- often also called complex fluids -- that display a large response to weak external perturbations and that possess properties governed by slow internal dynamics. Flowing matter refers to all systems that can actually flow, from simple to multiphase liquids, from foams to granular matter. Living matter concerns the new physics that emerges from novel insights into the properties and behaviours of living systems. Furthermore, it aims at developing new concepts and quantitative approaches for the study of biological phenomena. Approaches from soft matter physics and statistical physics play a key role in this research. The journal includes reports of experimental, computational and theoretical studies and appeals to the broad interdisciplinary communities including physics, chemistry, biology, mathematics and materials science.