Photobiomodulation Activates Microglia/Astrocytes and Relieves Neuropathic Pain in Inferior Alveolar Nerve Injury.

IF 1.8 Q2 SURGERY
Erina Daigo, Yuki Daigo, Jun Idogaki, Hiroshi Fukuoka, Nobuko Fukuoka, Masatsugu Ishikawa, Kazuya Takahashi
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引用次数: 0

Abstract

Objective: This study aimed to determine microglial/astrocyte changes and their associated analgesic effect in inferior alveolar nerve injury (IANI) model rats treated with photobiomodulation therapy (PBMT) using a 940-nm diode laser. Background: Very few basic studies have investigated microglial/astrocyte dynamics following PBMT aimed at relieving neuropathic pain caused by IANI. Methods: Rats were divided into an IANI-PBM group, IANI+PBM group, and sham+PBM group. Observations were made on the day before IANI or the sham operation and on postoperative days 3, 5, 7, 14, and 28. PBMT was delivered for 7 consecutive days, with an energy density of 8 J/cm2. Behavioral analysis was performed to determine pain thresholds, and immunohistological staining was performed for the microglia marker Iba1 and astrocyte marker glial fibrillary acidic protein, which are observed in the spinal trigeminal nucleus. Results: Behavioral analysis showed that the pain threshold returned to the preoperative level on postoperative day 14 in the IANI+PBM group, but decreased starting from postoperative day 1 and did not improve thereafter in the IANI-PBM group (p ≤ 0.001). Immunological analysis showed that microglial and astrocyte cell counts were similar in the IANI+PBM group and IANI-PBM group shortly after IANI (day 3), but the expression area was larger (p ≤ 0.001) and hypertrophy of microglia and astrocyte cell bodies and end-feet extension (i.e., indicators of activation) were more prominent in the IANI+PBM group. Conclusions: PBMT after IANI prevented hyperalgesia and allodynia by promoting glial cell activation shortly after injury.

光生物调节可激活小胶质细胞/星形胶质细胞并缓解下肺泡神经损伤的神经性疼痛
研究目的本研究旨在确定下牙槽神经损伤(IANI)模型大鼠在使用 940 纳米二极管激光进行光生物调节疗法(PBMT)治疗后的小胶质细胞/胃红细胞变化及其相关镇痛效果。背景:很少有基础研究调查了旨在缓解 IANI 引起的神经病理性疼痛的 PBMT 治疗后小胶质细胞/胃肠细胞的动态变化。研究方法将大鼠分为 IANI-PBM 组、IANI+PBM 组和假+PBM 组。在 IANI 或假手术前一天以及术后第 3、5、7、14 和 28 天进行观察。连续 7 天使用 PBMT,能量密度为 8 J/cm2。进行行为分析以确定疼痛阈值,并对脊髓三叉神经核中的小胶质细胞标记物 Iba1 和星形胶质细胞标记物胶质纤维酸性蛋白进行免疫组织学染色。结果行为分析表明,IANI+PBM 组的疼痛阈值在术后第 14 天恢复到术前水平,但 IANI-PBM 组的疼痛阈值从术后第 1 天开始下降,之后也没有改善(p ≤ 0.001)。免疫学分析表明,IANI+PBM 组和 IANI-PBM 组的小胶质细胞和星形胶质细胞数量在 IANI 后不久(第 3 天)相似,但 IANI+PBM 组的表达面积更大(p ≤ 0.001),小胶质细胞和星形胶质细胞的细胞体肥大和内足扩展(即活化指标)更突出。结论IANI 后的 PBMT 可在损伤后短期内促进神经胶质细胞的活化,从而预防痛觉减退和异动症。
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来源期刊
CiteScore
4.10
自引率
0.00%
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期刊介绍: Photobiomodulation, Photomedicine, and Laser Surgery Editor-in-Chief: Michael R Hamblin, PhD Co-Editor-in-Chief: Heidi Abrahamse, PhD
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