Topical erythritol combined with L-ascorbyl-2-phosphate inhibits staphylococcal growth and alleviates staphylococcal overgrowth in skin lesions of canine superficial pyoderma.

T Tochio, K Kawano, K Iyori, R Makida, Y Kadota, T Fujii, H Ishikawa, T Yasutake, A Watanabe, K Funasaka, Y Hirooka, K Nishifuji
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Abstract

Erythritol (ERT) and L-ascorbyl-2-phosphate (APS) are bacteriostatic, but their effects on staphylococcal skin infections remain unknown. We aimed to determine whether ERT combined with APS inhibits the growth of staphylococci that are commonly isolated from pyoderma skin lesions in dogs. We investigated the individual and combined effects of ERT and APS on the growth of Staphylococcus pseudintermedius, S. schleiferi, and S. aureus using turbidity assays in vitro. Skin lesions from 10 dogs with superficial pyoderma were topically treated with 5% ERT and 0.1% APS for 28 days, and swabbed skin samples were then analyzed using 16S rRNA amplicon sequencing and quantitative real-time PCR (qPCR). Results showed that ERT inhibited S. pseudintermedius growth regardless of harboring the mecA gene, and APS increased the inhibitory effects of ERT against S. pseudintermedius, S. schleiferi, and S. aureus in vitro. Moreover, combined ERT and APS decreased the prevalence of staphylococci on canine skin lesions at the genus level. The combination slightly increased the α-diversity but did not affect the β-diversity of the microbiota. The qPCR results revealed that the combination significantly decreased S. pseudintermedius and S. schleiferi in skin lesions. Topical administration of EPS combined with APS can prevent staphylococcal colonization on the surface of mammalian skin. The results of this study may provide an alternative to systemic antibiotics for treating superficial pyoderma on mammalian skin surfaces.

外用赤藓糖醇联合 L-抗坏血酸-2-磷酸可抑制葡萄球菌生长,缓解犬浅表脓皮病皮损中葡萄球菌的过度生长。
赤藓糖醇(ERT)和L-抗坏血酸-2-磷酸酯(APS)具有抑菌作用,但它们对皮肤葡萄球菌感染的影响仍不清楚。我们的目的是确定 ERT 与 APS 结合使用是否能抑制通常从狗脓疱病皮肤病变中分离出的葡萄球菌的生长。我们使用体外浊度测定法研究了 ERT 和 APS 对假中间葡萄球菌、施莱佛里葡萄球菌和金黄色葡萄球菌生长的单独和联合作用。用 5%的 ERT 和 0.1% 的 APS 局部治疗 10 只患有浅表脓皮病的狗的皮损 28 天,然后用 16S rRNA 扩增子测序和定量实时 PCR(qPCR)分析拭取的皮肤样本。结果表明,无论是否携带 mecA 基因,ERT 都能抑制假金黄色葡萄球菌的生长,而 APS 则能增强 ERT 在体外对假金黄色葡萄球菌、Schleiferi 和金黄色葡萄球菌的抑制作用。此外,联合使用 ERT 和 APS 在属种水平上降低了犬皮损上葡萄球菌的感染率。联合使用可略微增加微生物群的α-多样性,但不会影响β-多样性。qPCR 结果表明,联合用药可显著减少皮损中的假金龟子和裂头蚴。EPS 与 APS 联合外用可防止哺乳动物皮肤表面的葡萄球菌定植。这项研究的结果可替代全身用抗生素治疗哺乳动物皮肤表面的浅表脓皮病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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