Expanding assay range to accommodate a monoclonal antibody therapeutic quantification in blood and cerebrospinal fluid.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-02-01 Epub Date: 2023-12-13 DOI:10.4155/bio-2023-0179

Alejandro R Foley, Gizette Sperinde, Saloumeh K Fischer

引用次数: 0

Abstract

Antibody therapeutic levels in neurodegenerative diseases are often measured in both serum and cerebrospinal fluid (CSF). Due to 0.1% drug partition from serum to CSF and the higher sensitivity needs, usually two different assays are required. The different Gyrolab Bioaffy compact discs can extend the dynamic range of assays. Here, an assay was developed and adapted on two different Gyrolab Bioaffy compact discs (200 and 4000 nl) to achieve the required sensitivity and assay dynamic range needed for the measurement of drug in both serum and CSF. This was accomplished by using the same critical reagents with minimal assay development to transition from a serum to a CSF assay.

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扩大检测范围，以适应血液和脑脊液中单克隆抗体的治疗定量。

神经退行性疾病的抗体治疗水平通常在血清和脑脊液（CSF）中同时测定。由于从血清到 CSF 的药物分配率为 0.1%，而且灵敏度要求较高，因此通常需要两种不同的检测方法。不同的 Gyrolab Bioaffy 光盘可以扩大检测的动态范围。在此，我们在两种不同的 Gyrolab Bioaffy 光盘（200 nl 和 4000 nl）上开发并调整了一种检测方法，以达到测量血清和 CSF 中药物所需的灵敏度和检测动态范围。这是通过使用相同的关键试剂实现的，从血清到脑脊液检测只需进行最少的检测开发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.