{"title":"Precision genetic technologies for cereal functional genomics","authors":"","doi":"10.1007/s13562-023-00862-0","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> <p>Identifying and characterizing genes that control important agronomic traits and finding ways to alter them are vital for crop improvement. Map-based cloning, heterologous gene expression, RNAi mediated gene silencing, T-DNA insertional mutation and TILLING technologies have enabled cloning, characterization, and deployment of a few important genes. Revolution in sequencing technologies and bioinformatics has facilitated us to quickly predict and annotate plethora of genes and regulatory elements with improved precision and spatio-temporal expression of genes in almost all cereal crops. However, their functional validation forms a bottleneck in exploiting useful genetic elements for crop improvement. The CRISPR/Cas9 mediated gene editing has become the tool of choice for precisely introducing targeted modifications in the genome to knock out/in a gene, introducing specific mutation in sequence or modulating its expression in diverse crop species. This can help to rapidly characterize plenty of genes in terms of understanding the function of individual gene/ gene family, involvement in a particular biochemical pathway or interaction of the crop plant with external stimuli at a reasonable cost. In this review, we discuss the power of gene editing for rapid functional characterization of genetic elements as a fundamental requirement to harness the power of precision genetic technologies for increasing crop yield, progress made so far, opportunities and challenges.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s13562-023-00862-0","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Identifying and characterizing genes that control important agronomic traits and finding ways to alter them are vital for crop improvement. Map-based cloning, heterologous gene expression, RNAi mediated gene silencing, T-DNA insertional mutation and TILLING technologies have enabled cloning, characterization, and deployment of a few important genes. Revolution in sequencing technologies and bioinformatics has facilitated us to quickly predict and annotate plethora of genes and regulatory elements with improved precision and spatio-temporal expression of genes in almost all cereal crops. However, their functional validation forms a bottleneck in exploiting useful genetic elements for crop improvement. The CRISPR/Cas9 mediated gene editing has become the tool of choice for precisely introducing targeted modifications in the genome to knock out/in a gene, introducing specific mutation in sequence or modulating its expression in diverse crop species. This can help to rapidly characterize plenty of genes in terms of understanding the function of individual gene/ gene family, involvement in a particular biochemical pathway or interaction of the crop plant with external stimuli at a reasonable cost. In this review, we discuss the power of gene editing for rapid functional characterization of genetic elements as a fundamental requirement to harness the power of precision genetic technologies for increasing crop yield, progress made so far, opportunities and challenges.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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