Thermodynamic study on hydrated bilayers of ether-linked phosphatidylcholines with terminal perfluorobutyl group

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2023-12-14 DOI:10.1016/j.bbamem.2023.184261

Masaya Miyazaki , Chika Arisaka , Ai Nakagawara , Nanako Sasaki , Hiroshi Takahashi , Toshiyuki Takagi , Hideki Amii , Masashi Sonoyama

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Abstract

Novel terminally perfluorobutyl group-containing ether-linked phosphatidylcholines with different alkyl chain lengths (di-O-F4-Cn-PCs, n = 14,16 and 18) were developed as possible materials for stable liposomes aiming at applications of structural and functional analyses of membrane proteins. Differential scanning calorimetric investigations of the thermotropic transition of hydrated di-O-F4-Cn-PC bilayers demonstrated that the transition temperature of every di-O-F4-Cn-PC decreases by ~20 °C compared to their corresponding non-fluorinated PCs, di-O-Cn-PCs. With the elongation of the hydrophobic chain, on the other hand, the transition enthalpy (ΔH) and entropy (ΔS) increased in a linear manner. Comparison of ΔH and ΔS values against the net hydrocarbon chain length between di-O-F4-Cn-PCs and di-O-Cn-PCs strongly suggests that in the thermotropic transition of the di-O-F4-Cn-PC membrane, the perfluorobutyl segments undergo very limited structural changes; therefore, the hydrocarbon segments are mainly responsible for the phase transition.

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带有末端全氟丁基的醚键磷脂酰胆碱水合双分子层的热力学研究

研究人员开发了具有不同烷基链长的新型末端含全氟丁基的醚键磷脂酰胆碱（di-O-F4-Cn-PCs，n = 14、16 和 18），作为稳定脂质体的可能材料，旨在应用于膜蛋白的结构和功能分析。对水合二-O-F4-Cn-PC 双分子层的向热转变进行的差示扫描量热研究表明，与相应的非氟 PCs（二-O-Cn-PCs）相比，每种二-O-F4-Cn-PC 的转变温度降低了约 20 ℃。另一方面，随着疏水链的延长，转化焓（ΔH）和转化熵（ΔS）呈线性增长。将二-O-F4-Cn-PCs 和二-O-Cn-PCs 的 ΔH 和 ΔS 值与碳氢链净长度进行比较，可有力地表明在二-O-F4-Cn-PC 膜的热致转变过程中，全氟丁基段的结构变化非常有限；因此，碳氢段是造成相变的主要原因。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464