First report of the whole-genome sequence analysis of avian rotavirus A from Japanese chickens

IF 1.9 4区 医学 Q3 GENETICS & HEREDITY
Ayana Okamoto, Hitoshi Takemae, Makoto Nagai, Shinichiro Hashimoto, Tetsuya Mizutani, Tetsuya Furuya
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引用次数: 0

Abstract

Rotavirus A infects many mammalian species, including humans and causes diarrhea and gastrointestinal diseases. The virus also infects various bird species, including chickens, although information of avian rotavirus A (ARVA) infection in chicken populations in Japan is scarce. In this study, we report for the first time the whole-genome sequences of ARVA strains from Japanese chicken populations. The virus strains were inoculated to MA104 cells and cultured viruses were used to obtain the sequences with the MiSeq system, and genetic analysis demonstrated the genotype constellation of G19-P[30]-I11-R6-C6-M7-A16-N6-T8-E10-H8 of the Japanese chicken ARVA isolates. Phylogenetic analyses demonstrated that the VP1, VP2, VP3, VP4, VP7, NSP2, and NSP4 coding gene sequences of the Japanese strains were closer to those of Korean than the European ARVA strains, although such relationship was not clear for other genes. The data suggest that the Japanese ARVA strains and the ones in Korea have genetically close relationship, although the origin is not clear at this point. Further information including the whole-genome sequences of the Korean strains and sequences of other Japanese chicken ARVA strains will be necessary for elucidation of their origin.

Abstract Image

首次报告日本鸡禽类轮状病毒 A 的全基因组序列分析
轮状病毒 A 可感染包括人类在内的多种哺乳动物,并导致腹泻和胃肠道疾病。该病毒也会感染包括鸡在内的各种鸟类,但日本鸡群感染禽轮状病毒 A(ARVA)的信息却很少。在本研究中,我们首次报告了日本鸡群中 ARVA 株系的全基因组序列。将病毒株接种到 MA104 细胞中,利用 MiSeq 系统获得培养病毒的序列,遗传分析表明日本鸡群 ARVA 分离株的基因型为 G19-P[30]-I11-R6-C6-M7-A16-N6-T8-E10-H8 型。系统发育分析表明,日本毒株的 VP1、VP2、VP3、VP4、VP7、NSP2 和 NSP4 编码基因序列与韩国毒株的序列比较接近,而与欧洲 ARVA 毒株的序列比较接近,但与其他基因的关系不明显。这些数据表明,日本的 ARVA 株系与韩国的 ARVA 株系在遗传学上关系密切,但起源目前尚不清楚。要弄清这些菌株的来源,还需要更多的信息,包括韩国菌株的全基因组序列和其他日本鸡 ARVA 菌株的序列。
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来源期刊
Virus Genes
Virus Genes 医学-病毒学
CiteScore
3.30
自引率
0.00%
发文量
76
审稿时长
3 months
期刊介绍: Viruses are convenient models for the elucidation of life processes. The study of viruses is again on the cutting edge of biological sciences: systems biology, genomics, proteomics, metagenomics, using the newest most powerful tools. Huge amounts of new details on virus interactions with the cell, other pathogens and the hosts – animal (including human), insect, fungal, plant, bacterial, and archaeal - and their role in infection and disease are forthcoming in perplexing details requiring analysis and comments. Virus Genes is dedicated to the publication of studies on the structure and function of viruses and their genes, the molecular and systems interactions with the host and all applications derived thereof, providing a forum for the analysis of data and discussion of its implications, and the development of new hypotheses.
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