A comprehensive LC-MS/MS method for simultaneous analysis of 65 synthetic cannabinoids in human hair samples and application to forensic investigations

IF 1.2 4区医学 Q3 MEDICINE, LEGAL

Journal of forensic and legal medicine Pub Date : 2023-12-15 DOI:10.1016/j.jflm.2023.102636

Yiqi Yang , Buyi Xu , Daoxia Li , Qifu Zhang , Jing Zhang , Lin Yang , Yi Ye

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引用次数: 0

Abstract

Synthetic cannabinoids (SCs) represent a diverse class of new psychoactive substances characterized by extensive substance variety and severe abuse implications. The current situation of synthetic cannabinoid abuse in China is getting worse, with an increasing number of SC variants emerging. Therefore, it is imperative to improve synthetic cannabinoid detecting methods to align with the prevalent abuse situation in the region. In this study, a reliable and validated liquid chromatography-tandem mass spectrometry method was developed for the qualitative and quantitative analysis of 65 SC analogues in human hair samples. The validation results demonstrated satisfactory linearity (r ≥ 0.99) within the range of 25–2500 pg/mg for each SC analogue. The method exhibited limits of detection ranging from 10 to 15 pg/mg and limits of quantification ranging from 25 to 40 pg/mg. The relative standard deviations of intra-day precision and inter-day precision were below 15 %. Furthermore, negligible matrix effects were observed, with recovery rates ranging from 85.70 % to 119.43 %. Analysis of abuser demographics revealed that the primary group engaged in SC analogue abuse consisted of adolescents, predominantly males, accounting for 79.5 % of cases. Among the suspected individuals, ADB-BUTINACA and MDMB-4en-PINACA were the most frequently detected substances. The present study develops a highly sensitive analytical method and provides a comprehensive overview of the prevalence of SC abuse in the eastern region of China.

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同时分析人类毛发样本中 65 种合成大麻素的综合 LC-MS/MS 方法及其在法医调查中的应用

合成大麻素（SC）是一类多样化的新型精神活性物质，具有物质种类繁多、滥用危害严重的特点。我国合成大麻素滥用现状日益严重，合成大麻素变种不断涌现。因此，必须改进合成大麻素检测方法，以适应该地区普遍存在的滥用情况。本研究建立了一种可靠、有效的液相色谱-串联质谱（LC-MS/MS）方法，用于定性和定量分析人体毛发样品中的 65 种 SC 类似物。验证结果表明，每种 SC 类似物在 25-2500 pg/mg 范围内的线性关系良好（r ≥ 0.99）。该方法的检出限（LOD）为 10-15 pg/mg，定量限（LOQ）为 25-40 pg/mg。日内精密度和日间精密度的相对标准偏差均低于 15%。此外，基质效应可忽略不计，回收率在 85.70 % 到 119.43 % 之间。对滥用者人口统计学特征的分析表明，滥用 SC 类似物的主要群体是青少年，以男性为主，占病例总数的 79.5%。在可疑人员中，ADB-BUTINACA 和 MDMB-4en-PINACA 是最常检测到的物质。本研究开发了一种高灵敏度的分析方法，全面概述了中国东部地区合成大麻素滥用的流行情况。

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来源期刊

Journal of forensic and legal medicine MEDICINE, LEGAL-

CiteScore

2.70

自引率

6.70%

发文量

106

审稿时长

57 days

期刊介绍： The Journal of Forensic and Legal Medicine publishes topical articles on aspects of forensic and legal medicine. Specifically the Journal supports research that explores the medical principles of care and forensic assessment of individuals, whether adult or child, in contact with the judicial system. It is a fully peer-review hybrid journal with a broad international perspective. The Journal accepts submissions of original research, review articles, and pertinent case studies, editorials, and commentaries in relevant areas of Forensic and Legal Medicine, Context of Practice, and Education and Training. The Journal adheres to strict publication ethical guidelines, and actively supports a culture of inclusive and representative publication.