Thiobarbituric Acid Reactive Substances (TBARS) in Dog’s Hair as a Sign of Oxidative Stress – Preliminary Study

Nikolina Boić, Branimir Hackenberger Kutuzović, Davorka Hackenberger Kutuzović
{"title":"Thiobarbituric Acid Reactive Substances (TBARS) in Dog’s Hair as a Sign of Oxidative Stress – Preliminary Study","authors":"Nikolina Boić, Branimir Hackenberger Kutuzović, Davorka Hackenberger Kutuzović","doi":"10.2478/acve-2023-0034","DOIUrl":null,"url":null,"abstract":"Various endogenous and exogenous factors influence the occurrence of oxidative stress in all organisms, as well as in dogs. An increase in reactive oxygen species (ROS) concentration and the occurrence of oxidative stress can lead to changes in the structure of proteins, lipids, and DNA. The level of oxidative stress can be determined by measuring the end products of lipid peroxidation known as reactive substances of thiobarbituric acid (TBARS) of which malondialdehyde (MDA) is the most important. The concentration of MDA can be easily measured in various tissues and body excretions, but also by a non-invasive method of hair sampling. In this research, we have collected dog hair in grooming saloons, fluorometrically measured TBARS levels and compared the obtained values with factors such as breed, sex, age, passive smoking, sterilization, and season. No significant difference between sterilized and non-sterilized dogs was observed. The intensity of lipid peroxidation differed between the sexes, dog breeds, status of smoking by owner and exposure to UV radiation.","PeriodicalId":7379,"journal":{"name":"Acta Veterinaria","volume":"52 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Veterinaria","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/acve-2023-0034","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Various endogenous and exogenous factors influence the occurrence of oxidative stress in all organisms, as well as in dogs. An increase in reactive oxygen species (ROS) concentration and the occurrence of oxidative stress can lead to changes in the structure of proteins, lipids, and DNA. The level of oxidative stress can be determined by measuring the end products of lipid peroxidation known as reactive substances of thiobarbituric acid (TBARS) of which malondialdehyde (MDA) is the most important. The concentration of MDA can be easily measured in various tissues and body excretions, but also by a non-invasive method of hair sampling. In this research, we have collected dog hair in grooming saloons, fluorometrically measured TBARS levels and compared the obtained values with factors such as breed, sex, age, passive smoking, sterilization, and season. No significant difference between sterilized and non-sterilized dogs was observed. The intensity of lipid peroxidation differed between the sexes, dog breeds, status of smoking by owner and exposure to UV radiation.
狗毛发中作为氧化应激标志的硫代巴比妥酸活性物质 (TBARS) - 初步研究
各种内源性和外源性因素都会影响氧化应激在所有生物体和狗体内的发生。活性氧(ROS)浓度的增加和氧化应激的发生会导致蛋白质、脂质和 DNA 结构的改变。氧化应激的程度可以通过测量脂质过氧化的最终产物--硫代巴比妥酸活性物质(TBARS)来确定,其中丙二醛(MDA)是最重要的物质。MDA 的浓度很容易在各种组织和身体排泄物中测量,也可以通过毛发取样这种非侵入性方法测量。在这项研究中,我们在美容院采集了狗的毛发,用荧光计测量了 TBARS 水平,并将所得数值与品种、性别、年龄、被动吸烟、绝育和季节等因素进行了比较。结果表明,绝育犬和未绝育犬之间没有明显差异。脂质过氧化的强度在性别、狗的品种、主人吸烟情况和暴露于紫外线辐射下的情况之间存在差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信