Photo-activatable Reagents for Bioorthogonal Ligation Reactions

IF 8.6 2区化学 Q1 Chemistry

Topics in Current Chemistry Pub Date : 2023-12-13 DOI:10.1007/s41061-023-00447-4

Heyang Zhang, Ming Fang, Qing Lin

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Abstract

Light-induced bioorthogonal reactions offer spatiotemporal control over selective biomolecular labeling. This review covers the recent advances in the design of photo-activatable reagents for bioorthogonal conjugation reactions in living systems. These reagents are stable in the absence of light, but transformed into reactive species upon light illumination, which then undergo rapid ligation reactions. The light wavelength has been tuned from ultraviolet to near infrared to enable efficient photo-activation in reactions in deep tissues. The most prominent photo-activatable reagents are presented, including tetrazoles, tetrazines, 9,10-phenanthrenequinone, diarylsydnones, and others. A particular focus is on the strategies for improving reaction kinetics and biocompatibility accomplished through careful molecular engineering. The utilities of these photo-activatable reagents are illustrated through a broad range of biological applications, including in vivo protein labeling, positron emission tomography (PET) imaging, responsive hydrogels, and fluorescence microscopy. The further development and optimization of these biocompatible photo-activatable reagents should lead to new chemical biology strategies for studying biomolecular structure and function in living systems.

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用于生物正交连接反应的光活化试剂

光诱导的生物正交反应提供了对选择性生物分子标记的时空控制。本文综述了近年来用于生物正交偶联反应的光活化试剂的设计研究进展。这些试剂在没有光的情况下是稳定的，但在光照下转化为反应物质，然后进行快速的结扎反应。光的波长已经从紫外线调到近红外线，以便在深层组织的反应中进行有效的光激活。介绍了最突出的光活化试剂，包括四唑、四嗪、9,10-菲醌、二芳基酮等。一个特别的重点是改善反应动力学和生物相容性的策略，通过仔细的分子工程完成。这些光活化试剂的效用通过广泛的生物应用来说明，包括体内蛋白质标记，正电子发射断层扫描(PET)成像，反应性水凝胶和荧光显微镜。这些生物相容性光活化试剂的进一步开发和优化将为研究生命系统中的生物分子结构和功能提供新的化学生物学策略。

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来源期刊

Topics in Current Chemistry 化学-化学综合

CiteScore

11.70

自引率

1.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Topics in Current Chemistry provides in-depth analyses and forward-thinking perspectives on the latest advancements in chemical research. This renowned journal encompasses various domains within chemical science and their intersections with biology, medicine, physics, and materials science. Each collection within the journal aims to offer a comprehensive understanding, accessible to both academic and industrial readers, of emerging research in an area that captivates a broader scientific community. In essence, Topics in Current Chemistry illuminates cutting-edge chemical research, fosters interdisciplinary collaboration, and facilitates knowledge-sharing among diverse scientific audiences.