Effects of Ammonia Nitrogen, Nitrite and pH on Embryo In Vitro Incubation of Red Crayfish

Abstract

As an important economic aquatic animal, red crayfish’s reproduction and incubation can affect its breeding efficiency. With aquaculture developing, the study of factors affecting aquaculture species growth has gradually become an important research direction. To study the effects of ammonia nitrogen, nitrite and pH on red crayfish embryos, the In Vitro incubation experiment of red crayfish embryos was conducted. The residual module was used to design the embryo counting method of red crayfish. And different ammonia nitrogen, nitrite and PH gradients were set to analyze the hatching rate and emergence rate of red crayfish’s embryo development.When conducting research method performance testing, the training loss value of the research method decreased to 84 after 200 iterations. On the 6th day in an ammonia nitrogen environment, the number of water microbiota was only 4.74 M. The emergence rate of red crayfish decreases to a lower level when the ammonia nitrogen concentration is above 0.6 mg/L. The emergence rate of red crayfish decreased with the increase of nitrite concentration, and decreased to 0 when the nitrite concentration reached 1.25 mg/L. When the pH value of water body is 8.1, the emergence rate of red crayfish reaches the highest value of 68.92%. Ammonia nitrogen and nitrite concentration will inhibit the In Vitro hatching of crayfish embryos, and the appropriate PH value can improve the emergence rate of crayfish hatching In Vitro.