Emerging Sensing and In Situ Detection Technologies for the Analysis of Extracellular Vesicle miRNAs

IF 4 Q2 ENGINEERING, BIOMEDICAL

Advanced Nanobiomed Research Pub Date : 2023-12-03 DOI:10.1002/anbr.202300067

Jixuan Han, Chen Wang, Ling Zhu, Yanlian Yang

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Abstract

Liquid biopsy has received increasing attention as a new disease detection modality because of its noninvasive, simple sampling, and reproducible assay advantages. Among the markers of liquid biopsy, extracellular vesicles (EVs) are considered as promising disease biomarkers because they contain a large amount of biological information and have a significant role in physiological activities. The emergence and progression of some of these diseases are associated with miRNAs carried by EVs (EV-miRNAs). Therefore, high-sensitive detection of EV-miRNAs is essential in clinical applications. A growing number of strategies, including biosensors, in situ detection methods, and microfluidics have been developed for the detection of EV-miRNA and have been applied in the diagnosis of diseases such as cancer. This review summarizes the probes, signal amplification, and detection methods for EV-miRNA detection, as well as the application of membrane fusion-based in situ detection and integrated microfluidic chips for EV-miRNA detection. The challenges of these materials and techniques in clinical diagnostic applications are also discussed.

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用于分析细胞外囊泡 miRNA 的新兴传感和原位检测技术

液体活检作为一种新的疾病检测方式，因其无创、取样简单、可重复性强等优点而受到越来越多的关注。在液体活检标志物中，细胞外囊泡(extracellular vesicles, EVs)因其含有大量的生物学信息，在生理活动中具有重要作用，被认为是有前景的疾病生物标志物。其中一些疾病的发生和进展与EV携带的miRNAs (EV‐miRNAs)有关。因此，高灵敏度的EV - mirna检测在临床应用中至关重要。越来越多的策略，包括生物传感器、原位检测方法和微流体已经被开发用于检测EV - miRNA，并已应用于癌症等疾病的诊断。本文综述了EV - miRNA检测的探针、信号放大和检测方法，以及基于膜融合的原位检测和集成微流控芯片在EV - miRNA检测中的应用。这些材料和技术在临床诊断应用的挑战也进行了讨论。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advanced Nanobiomed Research nanomedicine, bioengineering and biomaterials-

CiteScore

5.00

自引率

5.90%

发文量

审稿时长

21 weeks

期刊介绍： Advanced NanoBiomed Research will provide an Open Access home for cutting-edge nanomedicine, bioengineering and biomaterials research aimed at improving human health. The journal will capture a broad spectrum of research from increasingly multi- and interdisciplinary fields of the traditional areas of biomedicine, bioengineering and health-related materials science as well as precision and personalized medicine, drug delivery, and artificial intelligence-driven health science. The scope of Advanced NanoBiomed Research will cover the following key subject areas: ▪ Nanomedicine and nanotechnology, with applications in drug and gene delivery, diagnostics, theranostics, photothermal and photodynamic therapy and multimodal imaging. ▪ Biomaterials, including hydrogels, 2D materials, biopolymers, composites, biodegradable materials, biohybrids and biomimetics (such as artificial cells, exosomes and extracellular vesicles), as well as all organic and inorganic materials for biomedical applications. ▪ Biointerfaces, such as anti-microbial surfaces and coatings, as well as interfaces for cellular engineering, immunoengineering and 3D cell culture. ▪ Biofabrication including (bio)inks and technologies, towards generation of functional tissues and organs. ▪ Tissue engineering and regenerative medicine, including scaffolds and scaffold-free approaches, for bone, ligament, muscle, skin, neural, cardiac tissue engineering and tissue vascularization. ▪ Devices for healthcare applications, disease modelling and treatment, such as diagnostics, lab-on-a-chip, organs-on-a-chip, bioMEMS, bioelectronics, wearables, actuators, soft robotics, and intelligent drug delivery systems. with a strong focus on applications of these fields, from bench-to-bedside, for treatment of all diseases and disorders, such as infectious, autoimmune, cardiovascular and metabolic diseases, neurological disorders and cancer; including pharmacology and toxicology studies.