The Effect of Polyvalent Staphylococcus Vaccine on the Gene Expression of IL-8, IFN-γ, IL-1R and Phagocytes in Mice Spleen

Research in health science Pub Date : 2023-12-03 DOI:10.22158/rhs.v8n4p95

Meng Zhou, Chenfei Qu, Zhu Lei, Chunhua Bi, Xiao Jiang, Ting Liu, Qi’an Yue

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Abstract

Objectives: To study the effect of Polyvalent staphylococcus vaccine on cellular immune function in mice. Methods: Forty SD kunming species of mice were randomly divided into low dose group (500 million/ml bacteria), middle dose group (1 billion/ml bacteria), high dose group (2 billion/ml bacteria) and control group, Mice were immunized with different doses of Polyvalent staphylococcus vaccine. The expression of IL-8, IFN - γ and IL-1R genes in spleen tissues of mice in different doses of experimental group and control group were detected by RT-PCR, and the phagocytic effect of phagocytes in abdominal cavity of mice was detected by abdominal smear Results: Compared with the normal control group, the expression level of IL-8, IFN - γ, IL-1R gene and phagocytic function of spleen in the experimental group were significantly higher than those in the control group (P < 0.01), the effect in the middle dose group were the best. Conclusion: Polyvalent staphylococcus vaccine can significantly improve the expression level of IL-8, IFN - γ, IL-1R gene in mouse spleen, and enhance the phagocytic function of phagocytes, and its effect showed dose-dependent manner.

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多价葡萄球菌疫苗对小鼠脾脏中 IL-8、IFN-γ、IL-1R 和吞噬细胞基因表达的影响

目的:研究多价葡萄球菌疫苗对小鼠细胞免疫功能的影响。方法:将40只SD昆明种小鼠随机分为低剂量组(5亿个/ml细菌)、中剂量组(10亿个/ml细菌)、高剂量组(20亿个/ml细菌)和对照组，分别接种不同剂量的多价葡萄球菌疫苗。采用RT-PCR检测不同剂量实验组和对照组小鼠脾组织中IL-8、IFN - γ和IL-1R基因的表达，采用腹腔涂片法检测小鼠腹腔吞噬细胞的吞噬作用。与正常对照组比较，试验组大鼠脾脏IL-8、IFN - γ、IL-1R基因表达水平及吞噬功能均显著高于正常对照组(P < 0.01)，以中剂量组效果最好。结论:多价葡萄球菌疫苗可显著提高小鼠脾脏IL-8、IFN - γ、IL-1R基因的表达水平，增强吞噬细胞的吞噬功能，且其作用呈剂量依赖性。

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Research in health science

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