The transformation and genome editing of Pisum sativum: protocols and their modifications

Q3 Agricultural and Biological Sciences

Ecological genetics Pub Date : 2023-12-04 DOI:10.17816/ecogen567891

V. Tvorogova, E. A. Potsenkovskaia, Elena P. Efremova, Nikolai V. Kozlov, Veronika Y. Simonova, E. Y. Krasnoperova, Zakhar S. Konstantinov, Daria V. Yakovleva, Anastasiya M. Artemiuk, Lyudmila A. Lutova

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引用次数: 0

Abstract

Pea (Pisum sativum) is an important agricultural crop and a model object in various fields of plant research. At the same time, the genetic modification of pea is still a difficult task, which, apparently, is associated with its low ability to regenerate. There are a lot of different pea transformation protocols, however, for most of them, the transformation efficiency, i.e. the number of transgenic plants per explant, is extremely low. In addition, none of the protocols known to us has demonstrated itself to be universal, i.e. suitable for all varieties and lines of peas. We searched for studies on the transformation and regeneration of peas and systematized the data obtained. The resulting database made it possible to identify the most effective protocols for the transformation and regeneration ofP. sativum, as well as to analyze statistically the general features of the methods used, such as the source of the explant, the composition of the culture media, the duration of cultivation, and so on. We assume that our system for the analysis of publications devoted toin vitrocell cultures can also be used for similar data on other plant species. This research was supported by the Sirius University of Science and Technology project: PBB-RND-2243.

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豌豆的转化和基因组编辑：协议及其修改

豌豆(Pisum sativum)是一种重要的农业作物，是植物研究各个领域的典范对象。与此同时，豌豆的基因改造仍然是一项艰巨的任务，这显然与它的低再生能力有关。目前有很多不同的豌豆转化方案，但大多数方案的转化效率(即每个外植体的转基因植株数量)都非常低。此外，我们所知道的任何议定书都没有证明自己是普遍适用的，即适用于所有品种和品种的豌豆。我们检索了有关豌豆转化和再生的研究，并将所获得的数据系统化。由此产生的数据库可以确定p的转换和再生的最有效的协议。以及对所采用方法的一般特点进行统计分析，如外植体的来源、培养基的组成、培养的持续时间等。我们认为，我们的系统用于分析专门用于玻璃细胞培养的出版物，也可以用于其他植物物种的类似数据。这项研究得到了天狼星科技大学项目的支持:PBB-RND-2243。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ecological genetics Environmental Science-Ecology

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The journal Ecological genetics is an international journal which accepts for consideration original manuscripts that reflect the results of field and experimental studies, and fundamental research of broad conceptual and/or comparative context corresponding to the profile of the Journal. Once a year, the editorial Board reviews and, if necessary, corrects the rules for authors and the journal rubrics.