Detection of Minchinia mytili DNA in mussels (Mytilus galloprovincialis) after a mass mortality event in the Adriatic Sea

Q3 Veterinary

Veterinarska stanica Pub Date : 2023-12-06 DOI:10.46419/vs.55.4.10

I. G. Zupičić, D. Oraić, I. Arzul, L. Canier, Mathilde Noyer, B. Chollet, S. Zrnčić

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引用次数: 0

Abstract

We investigated a mass mortality event of Mediterranean mussels (Mytilus galloprovincialis) in the Novigrad Sea, Croatia during winter 2022/2023. The mortality rate was up to 70% and was initially linked to an influx of freshwater from the Zrmanja River. Despite mitigation measures that included placing mussels deeper to avoid the influence of freshwater, mortalities continued. Subsequent analysis of 64 samples using cytology, histolology and real-time PCR to exclude listed diseases, yielded negative results. PCR testing for Haplosporidium pinnae revealed the presence of a haplosporidian-like DNA resembling Minchinia mytili. In the study of predominant bacteria, Psychrobacter sp., Colwellia sp., and Vibrio splendidus were detected. Histological examination showed no haplosporidium structures, and in situ hybridisation with probes for detection of Haplosporiidae did not confirmed the presence of M. mytili in mussel tissue. However, our study reports the first detection of M. mytili DNA in the Adriatic Sea, emphasising the need for extensive research and further analysis to determine the exact cause of these mass mortality events and the origin of the haplosporidium DNA.

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亚得里亚海贻贝（Mytilus galloprovincialis）大规模死亡事件后在贻贝（Mytilus galloprovincialis）中检测到 Minchinia mytili DNA

我们调查了2022/2023年冬季克罗地亚诺维格拉德海地中海贻贝(Mytilus galloprovincialis)的大规模死亡事件。死亡率高达70%，最初与来自Zrmanja河的淡水流入有关。尽管采取了缓解措施，包括将贻贝放得更深以避免淡水的影响，但死亡人数仍在继续。随后使用细胞学、组织学和实时PCR对64个样本进行分析，以排除所列疾病，结果为阴性。对羽状单孢子虫进行PCR检测，发现存在一种类似于mytili Minchinia的单孢子虫样DNA。在优势菌的研究中，检出了冻干菌(Psychrobacter sp.)、科氏菌(Colwellia sp.)和脾弧菌(Vibrio脾)。组织学检查未发现单孢子虫结构，原位杂交检测单孢子虫科探针未证实贻贝组织中存在密氏芽孢杆菌。然而，我们的研究报告了首次在亚得里亚海检测到M. mytili DNA，强调需要进行广泛的研究和进一步的分析，以确定这些大规模死亡事件的确切原因和单孢子虫DNA的起源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinarska stanica Veterinary-Veterinary (all)

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： The goal of the journal is to provide an international platform for the publication of articles in the fields of veterinary and animal sciences, and biotechnology. The content of the journal is particularly dedicated to veterinary practitioners, but also to veterinary scientists and university professors, to encourage them to share their knowledge and experience on this platform. Manuscripts submitted to the journal may include: original scientific papers, review articles, short communications, professional articles, case reports, conference reports and literary records and reviews of new book either in Croatian or English languages.