Effect of resveratrol on SH-SY5Y cells studied by atomic force microscopy

IF 2.5 3区工程技术 Q1 MICROSCOPY

Micron Pub Date : 2023-12-13 DOI:10.1016/j.micron.2023.103577

Jiayao Yuan , Zuobin Wang , Jianjun Dong , Mingyan Gao , Fan Yang , Hao Sun

{"title":"Effect of resveratrol on SH-SY5Y cells studied by atomic force microscopy","authors":"Jiayao Yuan , Zuobin Wang , Jianjun Dong , Mingyan Gao , Fan Yang , Hao Sun","doi":"10.1016/j.micron.2023.103577","DOIUrl":null,"url":null,"abstract":"<div>In this paper, the effects of resveratrol on the viability, morphology, biomechanics and bioelectricity of SH-SY5Y cells were studied by atomic force microscopy. MTT assay showed that resveratrol had a dose effect on SH-SY5Y cells, and its activity was related to drug concentration and drug action time. With the increase of resveratrol concentration or the extension of action time, the activity of SH-SY5Y cells decreased obviously. Atomic force microscope (AFM) was employed to quantitatively analyze the physical changes of cells. AFM study shows that resveratrol can transform SH-SY5Y cells from spindle to sphere, and increase the cell height and decrease the cell adhesion. Also, the elastic modulus increases under the action of low concentration of resveratrol decreases under the action of high concentration of resveratrol, and the electric signal decreases. This study reveals the impact of resveratrol on SH-SY5Y cells from the biological and biophysical perspectives, which is helpful for a more comprehensive understanding of the interaction mechanism between resveratrol and SH-SY5Y cells. These techniques have potential applications in evaluating the effects of chemical substances on cells and screening targeted drugs.</div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Micron","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0968432823001750","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROSCOPY","Score":null,"Total":0}

引用次数: 0

Abstract

In this paper, the effects of resveratrol on the viability, morphology, biomechanics and bioelectricity of SH-SY5Y cells were studied by atomic force microscopy. MTT assay showed that resveratrol had a dose effect on SH-SY5Y cells, and its activity was related to drug concentration and drug action time. With the increase of resveratrol concentration or the extension of action time, the activity of SH-SY5Y cells decreased obviously. Atomic force microscope (AFM) was employed to quantitatively analyze the physical changes of cells. AFM study shows that resveratrol can transform SH-SY5Y cells from spindle to sphere, and increase the cell height and decrease the cell adhesion. Also, the elastic modulus increases under the action of low concentration of resveratrol decreases under the action of high concentration of resveratrol, and the electric signal decreases. This study reveals the impact of resveratrol on SH-SY5Y cells from the biological and biophysical perspectives, which is helpful for a more comprehensive understanding of the interaction mechanism between resveratrol and SH-SY5Y cells. These techniques have potential applications in evaluating the effects of chemical substances on cells and screening targeted drugs.

查看原文本刊更多论文

用原子力显微镜研究白藜芦醇对 SH-SY5Y 细胞的影响

本文利用原子力显微镜研究了白藜芦醇对SH-SY5Y细胞活力、形态、生物力学和生物电的影响。MTT试验表明，白藜芦醇对SH-SY5Y细胞有剂量效应，其活性与药物浓度和药物作用时间有关。随着白藜芦醇浓度的增加或作用时间的延长，SH-SY5Y细胞的活性明显降低。原子力显微镜（AFM）用于定量分析细胞的物理变化。原子力显微镜研究表明，白藜芦醇能使SH-SY5Y细胞从纺锤形转变为球形，并能增加细胞高度，降低细胞粘附性。同时，在低浓度白藜芦醇的作用下，弹性模量增大；在高浓度白藜芦醇的作用下，弹性模量减小，电信号降低。本研究从生物学和生物物理学角度揭示了白藜芦醇对SH-SY5Y细胞的影响，有助于更全面地了解白藜芦醇与SH-SY5Y细胞的相互作用机制。这些技术在评估化学物质对细胞的影响和筛选靶向药物方面具有潜在的应用价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Micron 工程技术-显微镜技术

CiteScore

4.30

自引率

4.20%

发文量

100

审稿时长

31 days

期刊介绍： Micron is an interdisciplinary forum for all work that involves new applications of microscopy or where advanced microscopy plays a central role. The journal will publish on the design, methods, application, practice or theory of microscopy and microanalysis, including reports on optical, electron-beam, X-ray microtomography, and scanning-probe systems. It also aims at the regular publication of review papers, short communications, as well as thematic issues on contemporary developments in microscopy and microanalysis. The journal embraces original research in which microscopy has contributed significantly to knowledge in biology, life science, nanoscience and nanotechnology, materials science and engineering.