Renewable biosynthesis of isoprene from wastewater through a synthetic biology approach: the role of individual organic compounds

IF 6.1 2区 环境科学与生态学 Q2 ENGINEERING, ENVIRONMENTAL
Min Yang, Xianghui Li, Weixiang Chao, Xiang Gao, Huan Wang, Lu Lu
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Abstract

The biosynthesis of isoprene offers a more sustainable alternative to fossil fuel-based approaches, yet its success has been largely limited to pure organic compounds and the cost remains a challenge. This study proposes a waste-to-wealth strategy for isoprene biosynthesis utilizing genetically engineered E. coli bacteria to convert organic waste from real food wastewater. The impact of organic compounds present in wastewater on E. coli growth and isoprene production was systematically investigated. The results demonstrated that with filtration pretreatment of wastewater, isoprene yield, and production achieved 115 mg/g COD and 7.1 mg/(L·h), respectively. Moreover, even without pretreatment, isoprene yield only decreased by ∼ 24%, indicating promising scalability. Glucose, maltose, glycerol, and lactate are effective substrates for isoprene biosynthesis, whereas starch, protein, and acetate do not support E. coli growth. The optimum C/N ratio for isoprene production was found to be 8:1. Furthermore, augmenting essential nutrients in wastewater elevated the isoprene yield increased to 159 mg/g COD. The wastewater biosynthesis significantly reduced the cost (44%–53% decrease, p-value < 0.01) and CO2 emission (46%–55% decrease, p-value < 0.01) compared with both sugar fermentation and fossil fuel–based refining. This study introduced a more sustainable and economically viable approach to isoprene synthesis, offering an avenue for resource recovery from wastewater.

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通过合成生物学方法从废水中可再生地生物合成异戊二烯:单个有机化合物的作用
异戊二烯的生物合成为基于化石燃料的方法提供了一种更可持续的替代方法,但其成功在很大程度上仅限于纯有机化合物,成本仍然是一个挑战。本研究提出了一种变废为宝的异戊二烯生物合成策略,利用基因工程改造的大肠杆菌来转化实际食品废水中的有机废物。研究人员系统地调查了废水中的有机化合物对大肠杆菌生长和异戊二烯生产的影响。结果表明,对废水进行过滤预处理后,异戊二烯产量和生产量分别达到 115 mg/g COD 和 7.1 mg/(L-h)。此外,即使不进行预处理,异戊二烯产量也只减少了 24%,这表明该技术具有良好的可扩展性。葡萄糖、麦芽糖、甘油和乳酸盐是异戊二烯生物合成的有效底物,而淀粉、蛋白质和醋酸盐则不支持大肠杆菌的生长。研究发现,异戊二烯生产的最佳 C/N 比为 8:1。此外,增加废水中的必需营养物质可将异戊二烯产量提高到 159 毫克/克 COD。与糖发酵和基于化石燃料的精炼相比,废水生物合成大大降低了成本(降低 44%-53%,p 值为 0.01)和二氧化碳排放量(降低 46%-55%,p 值为 0.01)。这项研究为异戊二烯合成引入了一种更具可持续性和经济可行性的方法,为从废水中回收资源提供了一条途径。
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来源期刊
Frontiers of Environmental Science & Engineering
Frontiers of Environmental Science & Engineering ENGINEERING, ENVIRONMENTAL-ENVIRONMENTAL SCIENCES
CiteScore
10.90
自引率
12.50%
发文量
988
审稿时长
6.1 months
期刊介绍: Frontiers of Environmental Science & Engineering (FESE) is an international journal for researchers interested in a wide range of environmental disciplines. The journal''s aim is to advance and disseminate knowledge in all main branches of environmental science & engineering. The journal emphasizes papers in developing fields, as well as papers showing the interaction between environmental disciplines and other disciplines. FESE is a bi-monthly journal. Its peer-reviewed contents consist of a broad blend of reviews, research papers, policy analyses, short communications, and opinions. Nonscheduled “special issue” and "hot topic", including a review article followed by a couple of related research articles, are organized to publish novel contributions and breaking results on all aspects of environmental field.
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