The Characteristics of Bacillus cereus Group Strains Isolated from Permafrost in Yakutia for Assessment of Microbiological Risks during Climate Change

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Biochemistry and Microbiology Pub Date : 2023-12-10 DOI:10.1134/s0003683823060054

Y. O. Goncharova, V. V. Evseeva, R. I. Mironova, K. V. Khlopova, A. G. Bogun, A. A. Sizova, V. I. Solomentsev, G. M. Titareva, I. V. Bahtejeva, T. B. Kravchenko, A. V. Brushkov, V. S. Timofeev, S. G. Ignatov

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Abstract

Strains of Bacillus genus were isolated from soil samples in the permafrost region (Yakutia, Russia). The phenotypic characteristics of the strains are given. The analysis of the obtained data made it possible to assign them to the Bacillus cereus complex. PCR analysis made it possible to determine the profile of B. cereus toxin synthesis genes in the genomes of the studied strains. Genetic characterization was obtained by RAPD genotyping and using MLVA loci used for genotyping of the anthrax pathogen. The results of genotyping at different levels of resolution made it possible to differentiate the studied strains from the B. anthracis species and to show their intraspecific genetic differences and the degree of relationship. Whole genome sequencing was carried out, based on the data of which MLST genotyping was carried out, which revealed two known sequence types and one new one that is described for the first time in this work. The results we obtained are of practical importance and are extremely interesting from the point of view of the evolution and phylogeography of the B. cereus group, since the fact that strains were isolated from permafrost suggests that their age may be much older than expected.

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从雅库特永久冻土中分离出的蜡样芽孢杆菌菌株的特征，用于评估气候变化期间的微生物风险

从永久冻土地区（俄罗斯雅库特）的土壤样本中分离出了芽孢杆菌属菌株。文中给出了菌株的表型特征。通过对所获数据的分析，可以将这些菌株归入蜡样芽孢杆菌复合菌种。通过 PCR 分析，确定了所研究菌株基因组中蜡样芽孢杆菌毒素合成基因的概况。通过 RAPD 基因分型和使用用于炭疽病原体基因分型的 MLVA 位点，获得了遗传特征。不同分辨率的基因分型结果可以将所研究的菌株与炭疽杆菌物种区分开来，并显示出它们的种内遗传差异和关系程度。我们进行了全基因组测序，并根据测序数据进行了 MLST 基因分型。我们获得的结果具有重要的现实意义，而且从蜡样芽孢杆菌群的进化和系统地理学的角度来看也非常有趣，因为从永久冻土中分离出菌株这一事实表明，它们的年龄可能比预期的要老得多。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.

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