Endochondral ossification of hindlimbs in embryonic development of Japanese Quail (Coturnix japonica)

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-12-01 DOI:10.1016/j.avrs.2023.100152

Xuan Li, Yuxin Zhang, Hongfeng Zhao

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引用次数: 0

Abstract

The endochondral ossification of hindlimb is essential to a bird's ability to stand, walk and fly. Most hindlimb is ossified in the embryos before hatching in precocial birds. However, the molecular mechanisms of hindlimb ossification in birds is still unclear. Therefore, we tried to examine the process of hindlimb ossification and its molecular regulation by using an animal model—Japanese Quail (Coturnix japonica). We selected four critical stages (Embryo Day: E6, E8, E12 and E16) of skeletal development of embryonic quails for hindlimb skeleton staining to show the process of endochondral ossification and to examine the molecular regulation of endochondral osteogenesis by RNA-Seq analysis. The results showed that ossification became increased with embryonic development and most hindlimb was ossified before hatching. RNA-Seq analysis revealed that various signaling pathways were involved with endochondral ossification with thyroid hormone signaling and WNT signaling pathway particularly enriched. Moreover, the expression levels of 42 genes were continuously upregulated and 14 genes were continuously downregulated from E6 to E16. The present study might provide new insights into complex molecular mechanisms in regulation of endochondral ossification.

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日本鹌鹑胚胎发育过程中后肢的软骨内骨化

后肢的软骨内骨化对鸟类的站立、行走和飞行能力至关重要。早熟鸟类的大部分后肢在孵化前的胚胎中就已经骨化。然而，鸟类后肢骨化的分子机制仍不清楚。因此，我们试图利用动物模型--日本鹌鹑（Coturnix japonica）来研究后肢骨化的过程及其分子调控。我们选择了胚胎鹌鹑骨骼发育的四个关键阶段（胚胎日：E6、E8、E12和E16）进行后肢骨骼染色，以显示软骨内骨化的过程，并通过RNA-Seq分析研究软骨内骨生成的分子调控。结果显示，骨化随着胚胎发育而增加，大部分后肢在孵化前就已经骨化。RNA-Seq分析显示，多种信号通路参与了软骨内骨化，其中甲状腺激素信号通路和WNT信号通路的表达尤其丰富。此外，从E6到E16，42个基因的表达水平持续上调，14个基因的表达水平持续下调。本研究可能会对调控软骨内骨化的复杂分子机制提供新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.