Investigation of tick-borne bacterial microorganisms in Haemaphysalis ticks from Hebei, Shandong, and Qinghai provinces, China

IF 3.1 2区 医学 Q2 INFECTIOUS DISEASES
Bing Zhang , Xiao Wang , Rewuzi Aguli·Nurland , Miao Lu , Yaqun Guan , Mengyun Liu , Fan Gao , Kun Li
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引用次数: 0

Abstract

Tick-borne microorganisms in many tick species and many areas of China are still not thoroughly investigated. In this study, 224 ticks including two species (Haemaphysalis longicornis and Haemaphysalis qinghaiensis) were collected from four cities in Hebei, Shandong, and Qinghai provinces, China. Ticks were screened for the presence of tick-borne bacterial microorganisms including Rickettsia, Anaplasmataceae (Anaplasma, Ehrlichia, Neoehrlichia, etc.), Coxiella, Borrelia, and Bartonella. Two Anaplasma species (Anaplasma ovis and Anaplasma capra) were detected in H. longicornis from Xingtai City of Hebei Province, with a positive rate of 3 % and 8 %, respectively. A Coxiella species was detected in H. longicornis ticks from all three locations in Hebei and Shandong provinces, with the positive rate ranging from 30 to 75 %. All the 16S and rpoB sequences were very similar (99.77–100 % identity) to Coxiella endosymbiont of Haemaphysalis ticks. An Ehrlichia species was detected in H. qinghaiensis (6/66, 9 %) from Xining City, Qinghai Province. The 16S and groEL sequences had 100 % and 97.40–97.85 % nucleotide identities to “Candidatus Ehrlichia pampeana” strains, respectively, suggesting that it may be a variant of “Candidatus Ehrlichia pampeana”. All the ticks were negative for Rickettsia, Borrelia, and Bartonella. Because all the ticks were removed from goats or humans and were partially or fully engorged, it is possible that the microorganisms were from the blood meal but not vectored by the ticks. Our results may provide some information on the diversity and distribution of tick-borne pathogens in China.

中国河北、山东和青海三省蜱传细菌微生物调查
中国许多地区和许多蜱类中的蜱传微生物仍未得到深入研究。本研究从中国河北、山东和青海省的四个城市采集了 224 只蜱虫,其中包括两个物种(长角蜱和青海蜱)。对蜱虫进行了细菌微生物筛查,包括立克次体、无形体科(无形体、埃利希体、新埃利希体等)、柯西氏菌、包柔氏菌和巴顿氏菌。在河北省邢台市的长角牛体内检测到两种阿纳普拉斯菌(羱羊阿纳普拉斯菌和羯羊阿纳普拉斯菌),阳性率分别为 3 % 和 8 %。河北和山东三地的长角蜱中都检测到了一种柯西氏菌,阳性率在 30% 到 75% 之间。所有的 16S 和 rpoB 序列与长角蜱的柯西氏菌内共生体非常相似(99.77%-100%)。在青海省西宁市的 H. qinghaiensis(6/66,9%)中检测到一种埃利希氏菌。16S和groEL序列与 "Candidatus Ehrlichia pampeana "菌株的核苷酸相同度分别为100%和97.40-97.85%,表明它可能是 "Candidatus Ehrlichia pampeana "的变种。所有蜱虫的立克次体、鲍氏杆菌和巴顿氏菌均为阴性。由于所有蜱虫都是从山羊或人身上取下的,而且部分或全部充血,因此微生物可能来自血餐,而不是由蜱虫传播。我们的研究结果可以为中国蜱传病原体的多样性和分布提供一些信息。
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来源期刊
Ticks and Tick-borne Diseases
Ticks and Tick-borne Diseases INFECTIOUS DISEASES-MICROBIOLOGY
CiteScore
6.90
自引率
12.50%
发文量
185
审稿时长
6-12 weeks
期刊介绍: Ticks and Tick-borne Diseases is an international, peer-reviewed scientific journal. It publishes original research papers, short communications, state-of-the-art mini-reviews, letters to the editor, clinical-case studies, announcements of pertinent international meetings, and editorials. The journal covers a broad spectrum and brings together various disciplines, for example, zoology, microbiology, molecular biology, genetics, mathematical modelling, veterinary and human medicine. Multidisciplinary approaches and the use of conventional and novel methods/methodologies (in the field and in the laboratory) are crucial for deeper understanding of the natural processes and human behaviour/activities that result in human or animal diseases and in economic effects of ticks and tick-borne pathogens. Such understanding is essential for management of tick populations and tick-borne diseases in an effective and environmentally acceptable manner.
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