Toward versatile digital bioanalysis

IF 2.6 4区工程技术 Q2 BIOCHEMICAL RESEARCH METHODS

Biomicrofluidics Pub Date : 2023-12-07 DOI:10.1063/5.0174727

Jun Ando, Rikiya Watanabe

引用次数: 0

Abstract

Digital bioanalysis places great emphasis on the highly sensitive and rapid detection of biomolecules at the single-molecule level. Rooted in single-molecule biophysics, this innovative approach offers numerous insights into biomolecular mechanisms with an unprecedented level of sensitivity and precision. Moreover, this method has significant potential to contribute to disease diagnostics, enabling the highly sensitive detection of biomarkers or pathogens for early disease diagnosis and continuous disease monitoring. However, the notable cost of detection and specialized equipment required for fabricating microdevices pose a challenge to accessibility and ease of use. This lack of versatility hinders the widespread adoption of digital bioanalysis. Here, we aim to illuminate the essential requirements for versatile digital bioanalysis and present prospects for biomedical applications that can be facilitated by attaining such versatility.

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实现多功能数字生物分析

数字生物分析非常重视在单分子水平上对生物分子进行高灵敏度的快速检测。这种创新方法植根于单分子生物物理学，能以前所未有的灵敏度和精确度洞察生物分子机制。此外，这种方法在疾病诊断方面也大有可为，可以高灵敏度地检测生物标志物或病原体，用于早期疾病诊断和持续疾病监测。然而，显著的检测成本和制造微型装置所需的专业设备对其可及性和易用性构成了挑战。这种多功能性的缺乏阻碍了数字生物分析技术的广泛应用。在此，我们旨在阐明多功能数字生物分析的基本要求，并介绍通过实现这种多功能性可促进生物医学应用的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomicrofluidics 生物-纳米科技

CiteScore

5.80

自引率

3.10%

发文量

审稿时长

1.3 months

期刊介绍： Biomicrofluidics (BMF) is an online-only journal published by AIP Publishing to rapidly disseminate research in fundamental physicochemical mechanisms associated with microfluidic and nanofluidic phenomena. BMF also publishes research in unique microfluidic and nanofluidic techniques for diagnostic, medical, biological, pharmaceutical, environmental, and chemical applications. BMF offers quick publication, multimedia capability, and worldwide circulation among academic, national, and industrial laboratories. With a primary focus on high-quality original research articles, BMF also organizes special sections that help explain and define specific challenges unique to the interdisciplinary field of biomicrofluidics. Microfluidic and nanofluidic actuation (electrokinetics, acoustofluidics, optofluidics, capillary) Liquid Biopsy (microRNA profiling, circulating tumor cell isolation, exosome isolation, circulating tumor DNA quantification) Cell sorting, manipulation, and transfection (di/electrophoresis, magnetic beads, optical traps, electroporation) Molecular Separation and Concentration (isotachophoresis, concentration polarization, di/electrophoresis, magnetic beads, nanoparticles) Cell culture and analysis(single cell assays, stimuli response, stem cell transfection) Genomic and proteomic analysis (rapid gene sequencing, DNA/protein/carbohydrate arrays) Biosensors (immuno-assay, nucleic acid fluorescent assay, colorimetric assay, enzyme amplification, plasmonic and Raman nano-reporter, molecular beacon, FRET, aptamer, nanopore, optical fibers) Biophysical transport and characterization (DNA, single protein, ion channel and membrane dynamics, cell motility and communication mechanisms, electrophysiology, patch clamping). Etc...