Strategy for Targeting Medical Diagnosis of Cerebral Ischemia Regions by Linking Gsk-3β Antibody and RVG29 to Magnetosomes

Abstract

Background: In our previous studies, we have identified Gsk-3β as a crucial target molecule in response to Danhong injection for cerebral ischemia intervention. Furthermore, it can serve as a molecular imaging probe for medical diagnosis. Bacterial magnetic particles (BMPs), synthesized by magnetotactic bacteria, are regarded as excellent natural nanocarriers. Methods: In this study, we utilized biological modification and chemical crosslinking techniques to produce a multifunctional BMP known as "RVG29-BMP-FA-Gsk-3β-Ab", which exhibits both magnetic properties and brain-targeting capabilities. Then, a combination of analytical techniques was used to characterize the properties of the multifunctional BMPs. Finally, we evaluated the cell targeting ability of the RVG29-BMP-FA-Gsk-3β-Ab. Results: The multifunctional BMPs were observed to possess uniform size and shape using TEM analysis, with a particle size of 70.1±7.33 nm. Zeta potential analysis revealed that the nanoparticles exhibited a regular and non-aggregative distribution of particle sizes. Relative fluorescence intensity results demonstrated that the complex of 1mg of RVG29-BMP-FA-Gsk- 3β-Ab could bind to FITC-RVG29 polypeptide at a concentration of 2189.5 nM. Cell viability analysis indicated its high biocompatibility and minimal cytotoxicity. The RVG29-BMP-FAGsk- 3β-Ab was observed to possess active targeting towards neuronal cells and fluorescence imaging capabilities in vitro, as evidenced by fluorescence imaging assays. The complex of RVG29-BMP-FA-Gsk-3β-Ab exhibited favourable properties for early diagnosis and efficacy evaluation of traditional Chinese medicine in treating cerebral ischemia. Conclusion: This study establishes a fundamental basis for the prospective implementation of multimodal imaging in traditional Chinese medicine for cerebral ischemia.