Arsenic (As)-Resistant Endophytic Bacteria Isolated from Ferns Growing in As-Contaminated Areas

IF 1.3 4区生物学 Q4 MICROBIOLOGY

Microbiology Pub Date : 2023-11-28 DOI:10.1134/s002626172260330x

T. B. K. Nguyen, T. H. T. Phan, T. T. Le, N. T. Dang, V. T. Nguyen, L. H. T. Nguyen, P. M. Nguyen

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Abstract

In this study, As-resistant endophytic bacteria (AEB) from four different ferns (i.e., Pteris vittata, Pityrogramma calomelanos, Blenchum orientale, and Nephrolepis exaltata) collected from the polymetallic mine (Nui Phao) in Vietnam were isolated and characterized. The sampling locations were contaminated with As concentration within the range of 316–1606 mg kg^–1. A total of 5 arsenite(III)- and 26 arsenate(V)-resistant endophytic bacterial strains that belonged to 13 different genera were obtained. The most predominant isolate (accounting for 36% of the total isolated AEB in root ferns) was identified as Bacillus sp. The isolates were more resistant to As(V) than to As(III). Among As(V)-resistant strains, 26 were resistant to As(V) at concentrations of 80–320 mM, whereas 5 As(III)-resistant strains were able to tolerate As(III) of up to 160 mM. Although all the isolates had the ability to produce indole acetic acid (IAA), only three strains (i.e., Sporosarcina luteola R3.3.1, Paenibacillus sp. R3.8.3, and Acinetobacter sp. L2.5.1) had the phosphate-solubilizing ability. The pot experiments using P. vittata and inoculation of the four selected AEB (i.e., Priestia megaterium R2.5.2, Micrococcus luteus S3.4.1, P. megaterium R3.4.5, and P. megaterium L3.5.1) confirmed the important role of AEB in plant growth promotion and phytoremediation potential of As-contaminated soils.

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砷污染地区蕨类植物中抗砷内生细菌的分离

摘要对越南Nui Phao多金属矿中4种蕨类植物(Pteris vittata、Pityrogramma calomelanos、Blenchum orientale和Nephrolepis exaltata)的抗砷内生细菌(AEB)进行了分离鉴定。采样点砷污染浓度范围为316 ~ 1606 mg kg-1。共获得耐亚砷酸盐(III)-内生细菌5株，耐亚砷酸盐(V)-内生细菌26株，分属13个属。结果表明，根蕨AEB的优势菌株为芽孢杆菌(Bacillus sp.)，占根蕨AEB分离株的36%。菌株对as (V)的抗性强于as (III)。在As(V)耐药菌株中，有26株对80 ~ 320 mM浓度的As(V)耐药，5株对160 mM浓度的As(III)耐药。虽然所有菌株都能产生吲哚乙酸(IAA)，但只有3株(luteola Sporosarcina R3.3.1、Paenibacillus sp. R3.8.3和Acinetobacter sp. L2.5.1)具有溶解磷酸盐的能力。通过盆栽试验和接种4种AEB(即:巨型葡萄球菌R2.5.2、黄体微球菌S3.4.1、巨型葡萄球菌R3.4.5和巨型葡萄球菌L3.5.1)，证实了AEB在促进植物生长和修复砷污染土壤中的重要作用。

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来源期刊

Microbiology 生物-微生物学

CiteScore

2.40

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Microbiology is an is an international peer reviewed journal that covers a wide range of problems in the areas of fundamental and applied microbiology. The journal publishes experimental and theoretical papers, reviews on modern trends in different fields of microbiological science, and short communications with descriptions of unusual observations. The journal welcomes manuscripts from all countries in the English or Russian language.