Morphology, molecular phylogeny and toxicity of Fukuyoa paulensis (Dinophyceae) from the Bay of Bengal, North Indian Ocean

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-12-04 DOI:10.1515/bot-2023-0023

Oyeshina G. Oyeku, Subir K. Mandal

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Abstract

Fukuyoa species are potential producers of polyether neurotoxins that are toxic to aquatic biota and humans. A strain of Fukuyoa (CSIRCSMCRI 004) was isolated from India’s Bay of Bengal coast. The clonal culture was identified using light and scanning electron microscopy and molecular phylogenetic analysis of the LSU rRNA gene and ITS (ITS1-5.8S gene-ITS2) sequences. Its toxicity was assessed using a 48 h Artemia bioassay. The morphological features observed included globular shape, descending cingular displacement, broad pentagonal 1′ plate, and large second apical plate (2′). The identity of the strain was confirmed to be F. paulensis based on the LSU rRNA gene and ITS sequences phylogenies. Strain CSIRCSMCRI 004 shared an identical sequence with the type strain of F. paulensis and other strains reported from different locations, based on the LSU rRNA gene and ITS sequences phylogenies. Exposure of brine shrimp larvae to its cellular lysate resulted in lethality (48 h LC50 = 0.256 mg ml−1) and behavioral abnormalities. The current study provides the first evidence of F. paulensis in the Bay of Bengal. It also reveals that CSIRCSMCRI 004 is toxic to susceptible biota like the brine shrimp and could produce ciguatera-associated toxins. The findings of this study highlight the need for sampling and screening of ciguatera-related species in the Bay of Bengal region to understand the associated public and environmental health risks.

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北印度洋孟加拉湾福古保利鱼的形态、分子系统发育和毒性

福冈物种是聚醚神经毒素的潜在生产者，对水生生物和人类有毒。从印度孟加拉湾沿岸分离出一株福古亚病毒(CSIRCSMCRI 004)。利用光镜和扫描电镜对克隆培养物进行鉴定，并对LSU rRNA基因和ITS (ITS1-5.8S基因- its2)序列进行分子系统发育分析。采用48 h青蒿素生物测定法评价其毒性。形态学特征为球状，呈下降的带状位移，宽的五边形1′板，大的第二尖板(2′)。通过LSU rRNA基因和ITS序列的系统发育分析，确认该菌株为保利梭菌。基于LSU rRNA基因和ITS序列的系统发育分析，菌株CSIRCSMCRI 004与F. paulensis型菌株和其他来自不同地点的菌株具有相同的序列。盐水对虾幼虫暴露于其细胞裂解液中导致死亡(48 h LC50 = 0.256 mg ml−1)和行为异常。目前的研究提供了第一个在孟加拉湾发现保利梭菌的证据。CSIRCSMCRI 004对卤虾等易感生物具有毒性，可产生雪卡水相关毒素。这项研究的结果突出表明，需要对孟加拉湾地区与雪克特有关的物种进行抽样和筛选，以了解相关的公共和环境健康风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.