Mineral trioxide aggregate in membrane form as a barrier membrane in guided bone regeneration

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences Pub Date : 2024-07-01 DOI:10.1016/j.jds.2023.11.021

Min-Yong Lee , Hi-Won Yoon , Si-Yoon Lee , Kwang-Mahn Kim , Su-Jung Shin , Jae-Sung Kwon

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引用次数: 0

Abstract

Background/purpose

In the field of conservative dentistry and endodontics, mineral trioxide aggregate (MTA), commonly used, possesses advantages such as biocompatibility, antimicrobial properties and osteogenic potential. This study investigated the feasibility of utilizing membrane form mineral trioxide aggregate (MTA) as a barrier membrane in guided bone regeneration (GBR) procedures.

Materials and methods

Membranes were electrospun from three different formulations: 15 w/v% Polycaprolactone (PCL), 13 w/v% PCL + 2 w/v% MTA (2MTA), and 11 w/v% PCL + 4 w/v% MTA (4MTA). Physicochemical and mechanical properties of the electrospun membrane were compared, encompassing parameters such as surface morphology, fiber diameter distribution, chemical composition, phase identification, tensile stress, pH variation, and water contact angle. Moreover, the antimicrobial properties against of the electrospun membranes were assessed through direct exposure to streptococcus aureus (S. aureus) and candida albicans (C. albicans). Additionally, on the 7th day, biocompatibility and cell attachment were investigated with respect to L929 (fibroblast) and MC3T3 (pre-osteoblast) cells. Inhibition of L929 cell infiltration and the expression of osteogenic related genes including osteocalcin (OCN), alkaline phosphatase (ALP), and runt related transcription factor 2 (RUNX2) in MC3T3 cells on 7th and 14th days were also investigated.

Results

PCL, 2MTA, and 4MTA exhibited no statistically differences in fiber diameter distribution and tensile stress. However, as the MTA content increased, wettability and pH also increased. Due to the elevated pH, 4MTA demonstrated the lowest viability S.aureus and C.albicans. All membranes were highly biocompatibility and promoted cell attachment, while effectively preventing L929 cell infiltration. Lastly 4MTA showed increase in OCN, ALP, and RUNX2 expression on both 7th and 14th day.

Conclusion

The membrane form MTA possessed characteristics essential for a novel barrier membrane.

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三氧化矿物以膜形式聚集，作为引导骨再生的屏障膜

背景/目的在保守牙科和牙髓学领域，矿物三氧化物聚集体(mineral trioxide aggregate, MTA)具有生物相容性、抗菌性和成骨潜能等优点，被广泛使用。本研究探讨了在引导骨再生(GBR)过程中利用膜状矿物三氧化骨料(MTA)作为屏障膜的可行性。用15 w/v%聚己内酯(PCL)、13 w/v% PCL + 2 w/v% MTA (2MTA)和11 w/v% PCL + 4 w/v% MTA (4MTA)三种不同的配方电纺膜。比较了电纺丝膜的物理化学和力学性能，包括表面形貌、纤维直径分布、化学成分、相识别、拉伸应力、pH变化和水接触角等参数。此外，通过直接暴露于金黄色链球菌(S. aureus)和白色念珠菌(C. albicans)，评估了静电纺丝膜的抗菌性能。此外，在第7天，研究了L929(成纤维细胞)和MC3T3(成骨前细胞)细胞的生物相容性和细胞附着。同时研究了MC3T3细胞第7、14天对L929细胞浸润及骨钙素(OCN)、碱性磷酸酶(ALP)、runt相关转录因子2 (RUNX2)等成骨相关基因表达的抑制作用。结果spcl、2MTA和4MTA在纤维直径分布和拉伸应力方面无统计学差异。随着MTA含量的增加，润湿性和pH值也随之增加。由于pH值升高，4MTA显示金黄色葡萄球菌和白色念珠菌的生存能力最低。所有膜均具有高度的生物相容性，促进细胞附着，同时有效阻止L929细胞浸润。最后，在第7天和第14天，4MTA均显示OCN、ALP和RUNX2的表达增加。结论MTA膜形态具有作为一种新型屏障膜所必需的特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Dental Sciences 医学-牙科与口腔外科

CiteScore

5.10

自引率

14.30%

发文量

348

审稿时长

6 days

期刊介绍： he Journal of Dental Sciences (JDS), published quarterly, is the official and open access publication of the Association for Dental Sciences of the Republic of China (ADS-ROC). The precedent journal of the JDS is the Chinese Dental Journal (CDJ) which had already been covered by MEDLINE in 1988. As the CDJ continued to prove its importance in the region, the ADS-ROC decided to move to the international community by publishing an English journal. Hence, the birth of the JDS in 2006. The JDS is indexed in the SCI Expanded since 2008. It is also indexed in Scopus, and EMCare, ScienceDirect, SIIC Data Bases. The topics covered by the JDS include all fields of basic and clinical dentistry. Some manuscripts focusing on the study of certain endemic diseases such as dental caries and periodontal diseases in particular regions of any country as well as oral pre-cancers, oral cancers, and oral submucous fibrosis related to betel nut chewing habit are also considered for publication. Besides, the JDS also publishes articles about the efficacy of a new treatment modality on oral verrucous hyperplasia or early oral squamous cell carcinoma.