P-hydroxybenzoic acid positively affect the Fusarium oxysporum to stimulate root rot in Panax notoginseng

IF 6.8 2区 医学 Q1 CHEMISTRY, MEDICINAL
Jing Zhao , Zhandi Wang , Rong Jiao , Qionglian Wan , Lianchun Wang , Liangxing Li , Yali Yang , Shahzad Munir
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引用次数: 0

Abstract

Background

Plant health is directly related to the change in native microbial diversity and changes in soil health have been implicated as one of the main cause of root rot. However, scarce information is present regarding allelopathic relationship of Panax notoginseng root exudates and pathogenic fungi Fusarium oxysporum in a continuous cropping system.

Methods

We analyzed P. notoginseng root exudate in the planting soil for three successive years to determine phenolic acid concentration using GC-MS and HPLC followed by effect on the microbial community assembly. Antioxidant enzymes were checked in the roots to confirm possible resistance in P. notoginseng.

Results

Total 29 allelochemicals in the planting soil extract was found with highest concentration (10.54 %) of p-hydroxybenzoic acid. The HPLC showing a year-by-year decrease in p-hydroxybenzoic acid content in soil of different planting years, and an increase in population of F. oxysporum. Moreover, community analysis displayed negative correlation with 2.22 mmol. L−1 of p-hydroxybenzoic acid correspond to an 18.1 % population of F. oxysporum. Furthermore, in vitro plate assay indicates that medium dose of p-hydroxybenzoic acid (2.5–5 mmol. L−1) can stimulate the growth of F. oxysporum colonies and the production of macroconidia, as well as cell wall-degrading enzymes. We found that 2–3 mmol. L−1 of p-hydroxybenzoic acid significantly increased the population of F. oxysporum.

Conclusion

In conclusion, our study suggested that p-hydroxybenzoic acid have negative effect on the root system and modified the rhizosphere microbiome so that the host plant became more susceptible to root rot disease.

Abstract Image

Abstract Image

对羟基苯甲酸正向影响尖孢镰刀菌对三七根系腐烂的刺激作用
植物的健康与本地微生物多样性的变化直接相关,土壤健康的变化被认为是根系腐烂的主要原因之一。然而,关于三七根系分泌物与病原菌尖孢镰刀菌化感作用关系的研究很少。方法连续3年对种植土壤中三七根分泌物进行气相色谱-质谱和高效液相色谱分析,测定其酚酸浓度,并分析其对微生物群落的影响。对三七根系的抗氧化酶进行了检测,以确定其可能的抗性。结果共检出29种化感物质,其中对羟基苯甲酸浓度最高(10.54 %)。HPLC结果显示,不同种植年限土壤对羟基苯甲酸含量呈逐年下降趋势,尖孢霉种群动态呈上升趋势。群落分析与2.22 mmol呈负相关。L−1的对羟基苯甲酸对应于18.1 %的尖孢镰刀菌种群。此外,体外平板实验表明,培养基中对羟基苯甲酸(2.5-5 mmol。L−1)可以促进尖孢菌菌落的生长和大分生孢子的产生,并促进细胞壁降解酶的产生。我们发现2-3 mmol。L−1对羟基苯甲酸能显著提高尖孢镰刀菌的种群数量。结论对羟基苯甲酸对植物根系有负面影响,并能改变根际微生物群,使寄主植物更易患根腐病。
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来源期刊
Journal of Ginseng Research
Journal of Ginseng Research CHEMISTRY, MEDICINAL-INTEGRATIVE & COMPLEMENTARY MEDICINE
CiteScore
11.40
自引率
9.50%
发文量
111
审稿时长
6-12 weeks
期刊介绍: Journal of Ginseng Research (JGR) is an official, open access journal of the Korean Society of Ginseng and is the only international journal publishing scholarly reports on ginseng research in the world. The journal is a bimonthly peer-reviewed publication featuring high-quality studies related to basic, pre-clinical, and clinical researches on ginseng to reflect recent progresses in ginseng research. JGR publishes papers, either experimental or theoretical, that advance our understanding of ginseng science, including plant sciences, biology, chemistry, pharmacology, toxicology, pharmacokinetics, veterinary medicine, biochemistry, manufacture, and clinical study of ginseng since 1976. It also includes the new paradigm of integrative research, covering alternative medicinal approaches. Article types considered for publication include review articles, original research articles, and brief reports. JGR helps researchers to understand mechanisms for traditional efficacy of ginseng and to put their clinical evidence together. It provides balanced information on basic science and clinical applications to researchers, manufacturers, practitioners, teachers, scholars, and medical doctors.
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