Saffron reduces the liver fibrosis in mice by inhibiting the JAK/STAT3 pathway.

Acta cirurgica brasileira Pub Date : 2023-12-01 eCollection Date: 2023-01-01 DOI:10.1590/acb385823

Lijuan Huang, Yan Han, Zhi Wang, Qiao Qiu, Sichen Yue, Qingmin Zhou, Wei Su, Jianhui Yan

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Abstract

Purpose: Chronic inflammation in the liver is a key trigger for liver injury and fibrosis in various liver diseases. Given the anti-inflammatory and antioxidant effects of Saffron, this study aimed to investigate the pharmacological effects of Saffron on hepatic inflammation and fibrosis.

Methods: The mice model of hepatic fibrosis was constructed using CCl4, and Saffron was administered at low (10 mg/kg) and high (20 mg/kg) doses by gavage. Then, the changes in liver function, liver inflammation and fibrosis markers were evaluated. The effects and mechanisms of Saffron on hepatic stellate cells were further investigated in in-vitro experiments.

Results: Saffron improved liver function, reduced liver inflammation and attenuated liver fibrosis in a dose-dependent manner in hepatic fibrosis mice. Furthermore, Western blotting showed that Saffron significantly inhibited JAK/STAT3 phosphorylation in fibrotic livers.

Conclusions: Saffron can attenuate liver fibrosis by inhibiting the JAK/STAT3 pathway and the activation of hepatic stellate cell, providing a theoretical basis for the development of new anti-fibrotic drugs.

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藏红花通过抑制JAK/STAT3通路减少小鼠肝纤维化。

目的:肝脏慢性炎症是各种肝脏疾病中肝损伤和纤维化的关键触发因素。鉴于藏红花具有抗炎和抗氧化作用，本研究旨在探讨藏红花对肝脏炎症和纤维化的药理作用。方法:用CCl4建立小鼠肝纤维化模型，灌胃低剂量(10 mg/kg)和高剂量(20 mg/kg)藏红花。然后，评估肝功能、肝脏炎症和纤维化指标的变化。通过体外实验进一步探讨藏红花对肝星状细胞的作用及其机制。结果:藏红花改善肝纤维化小鼠肝功能，减轻肝脏炎症，减轻肝纤维化，呈剂量依赖性。此外，Western blotting显示，藏红花可显著抑制纤维化肝脏中JAK/STAT3的磷酸化。结论:藏红花可通过抑制JAK/STAT3通路及肝星状细胞活化来减轻肝纤维化，为开发新型抗纤维化药物提供理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta cirurgica brasileira

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