Identification of Eimeria spp. in domestic chickens raised in alternative poultry production systems in the State of São Paulo, Brazil.

IF 1.3 4区农林科学 Q2 Veterinary

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2023-12-04 eCollection Date: 2023-01-01 DOI:10.1590/S1984-29612023075

José Carlos Soares Júnior, Bruno Ferraz Itoyama, Bruna Matarucco Sampaio Beretta, Camila Michele de Souza Hossotani, Maria Santa Cardoso Silva, Giane Serafim da Silva, Alex Akira Nakamura, Flávia Lombardi Lopes, Marcelo Vasconcelos Meireles

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Abstract

The objective of this study was to identify Eimeria spp. in alternative poultry production systems (APPS) in the State of São Paulo, Brazil. Fecal samples (168) and DNA extracted from fecal samples obtained in APPS located in different Municipalities in the State of São Paulo (93) were examined by microscopy or genera-specific PCR (ITS-1 locus). Samples positive for Eimeria spp. were examined using Eimeria lata, Eimeria nagambie, and Eimeria zaria species-specific PCR protocols (ITS-2 locus) and another E. lata-specific PCR (candidate IMP1 genomic locus) followed by molecular cloning (E. lata and E. zaria ITS-2 amplicons) and genetic sequencing. All positive DNA samples were also submitted to genera-specific nested PCR (18S rRNA gene) followed by next-generation sequencing to identify Eimeria spp. Eimeria nagambie, E. zaria, and Eimeria sp. were identified by ITS2-targeted species-specific PCRs and genetic sequencing. Next-generation sequencing identified, in order of prevalence: E. nagambie; Eimeria acervulina; Eimeria mivati; Eimeria praecox; Eimeria brunetti; Eimeria mitis; Eimeria sp.; Eimeria maxima; E. zaria, and Eimeria necatrix/tenella. Our results confirmed, for the first time in Brazil, the identification of E. nagambie, E. zaria, and Eimeria spp. ITS-2 and 18S rRNA gene sequences not yet described in Brazil.

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巴西圣保罗州替代家禽生产系统饲养的家鸡中艾美耳亚种的鉴定

本研究的目的是鉴定巴西圣保罗州替代家禽生产系统(APPS)中的艾美耳球虫。通过显微镜或属特异性PCR (ITS-1位点)检测了在圣保罗州(93)不同市的app中获得的168例粪便样本和提取的DNA。检测艾美耳球虫属阳性样品，采用拉塔艾美耳球虫、长山艾美耳球虫和扎利亚艾美耳球虫种特异性PCR (ITS-2位点)和另一种拉塔艾美耳球虫特异性PCR(候选IMP1基因组位点)，然后进行分子克隆(拉塔艾美耳球虫和扎利亚艾美耳球虫ITS-2扩增子)和基因测序。所有阳性DNA样本均进行属特异性巢式PCR (18S rRNA基因)鉴定，然后进行下一代测序鉴定艾美耳球虫，采用its2靶种特异性PCR和基因测序鉴定长山艾美耳球虫、扎利亚艾美耳球虫和艾美耳球虫。下一代测序确定(按流行程度排序):长崎肠杆菌;艾美球虫属acervulina;艾美球虫属mivati;艾美球虫属早熟;艾美球虫属brunetti;艾美球虫属轻的;艾美球虫属sp。艾美球虫属最大值;扎利埃氏绦虫和网状艾美耳虫/柔嫩艾美耳虫。我们的研究结果首次在巴西确认了在巴西尚未描述的纳加比埃默氏绦虫、扎里亚埃默氏绦虫和艾美耳亚种的ITS-2和18S rRNA基因序列。

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来源期刊

Revista Brasileira De Parasitologia Veterinaria PARASITOLOGY-VETERINARY SCIENCES

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： La revista es un órgano de difusión del Colegio Brasileño de Parasitología Veterinaria, con una especificidad dentro de esa área, la difusión de los resultados de la investigación brasileña en las áreas de Helmintología, Protozoología, Entomología y agentes transmitidos por artrópodos, relacionados con la salud animal.