Screening of potential key ferroptosis-related genes in Chronic Obstructive Pulmonary Disease.

IF 2.7 3区医学 Q2 RESPIRATORY SYSTEM

International Journal of Chronic Obstructive Pulmonary Disease Pub Date : 2023-12-01 eCollection Date: 2023-01-01 DOI:10.2147/COPD.S422835

Yumeng Cao, Huaqin Pan, Yanwei Yang, Jingrun Zhou, Guqin Zhang

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引用次数: 0

Abstract

Purpose: Ferroptosis plays essential roles in the development of COPD. We aim to identify the potential ferroptosis-related genes of COPD through bioinformatics analysis.

Methods: The RNA expression profile dataset GSE148004 was obtained from the GEO database. The ferroptosis-related genes were obtained from the FerrDb database. The potential differentially expressed ferroptosis-related genes of COPD were screened by R software. Then, protein-protein interactions (PPI), correlation analysis, gene-ontology (GO) enrichment analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were applied for the differentially expressed ferroptosis-related genes. Finally, hub gene-microRNA(miRNA), hug gene-transcription factor interaction networks were constructed by miRTarBase v8.0 and JASPAR respectively, and hub gene drugs were predicted by the Enrichr database.

Results: A total of 41 differentially expressed ferroptosis-related genes (22 up-regulated genes and 19 down-regulated genes) were identified between 7 COPD patients and 9 healthy controls. The PPI results demonstrated that these ferroptosis-related genes interacted with each other. The GO and KEGG enrichment analyses of differentially expressed ferroptosis-related genes indicated several enriched terms related to ferroptosis, central carbon metabolism in cancer, and the HIF-1 signaling pathway. The crucial miRNAs and drugs associated with the top genes were identified.

Conclusion: We identified 41 potential ferroptosis-related genes in COPD through bioinformatics analysis. HIF1A, PPARG, and KRAS may affect the development of COPD by regulating ferroptosis. These results may expand our understanding of COPD and might be useful in the treatment of COPD.

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慢性阻塞性肺疾病中潜在关键铁中毒相关基因的筛选。

目的:上睑下垂在慢性阻塞性肺病的发展中起重要作用。我们的目标是通过生物信息学分析来确定COPD的潜在铁中毒相关基因。方法:从GEO数据库中获取RNA表达谱数据集GSE148004。残铁相关基因从ferdb数据库中获得。利用R软件筛选COPD患者铁沉降相关基因的潜在差异表达。然后，对差异表达的凋亡相关基因进行蛋白-蛋白相互作用(PPI)、相关分析、基因本体(GO)富集分析和京都基因与基因组百科全书(KEGG)途径富集分析。最后，分别利用miRTarBase v8.0和JASPAR构建枢纽基因- microrna (miRNA)、拥抱基因-转录因子相互作用网络，并利用富集数据库对枢纽基因药物进行预测。结果:在7例COPD患者和9例健康对照者中共鉴定出41个差异表达的嗜铁相关基因(上调基因22个，下调基因19个)。PPI结果表明，这些嗜铁相关基因相互作用。对差异表达的铁中毒相关基因进行GO和KEGG富集分析，发现了与铁中毒、癌症中心碳代谢和HIF-1信号通路相关的几个富集项。鉴定了与顶级基因相关的关键mirna和药物。结论:通过生物信息学分析，我们鉴定出41个COPD中可能存在的铁中毒相关基因。HIF1A、PPARG和KRAS可能通过调节铁下垂影响COPD的发展。这些结果可能扩大我们对慢性阻塞性肺病的认识，并可能对慢性阻塞性肺病的治疗有用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Chronic Obstructive Pulmonary Disease RESPIRATORY SYSTEM-

CiteScore

4.80

自引率

10.70%

发文量

372

审稿时长

16 weeks

期刊介绍： An international, peer-reviewed journal of therapeutics and pharmacology focusing on concise rapid reporting of clinical studies and reviews in COPD. Special focus will be given to the pathophysiological processes underlying the disease, intervention programs, patient focused education, and self management protocols. This journal is directed at specialists and healthcare professionals