Exploring Potential Biomarkers of Early Thymoma based on Serum Proteomics.

IF 1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Protein and Peptide Letters Pub Date : 2024-01-01 DOI:10.2174/0109298665275655231103105924

Min Jin, Peng Liu, Guoyan Qi

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引用次数: 0

Abstract

Background: Early diagnosis remains difficult because the early symptoms of thymoma are atypical.

Objectives: This study aimed to analyze the changes of serum proteins in the early stage of thymoma (stage I/II) by proteomics method and to screen and validate candidate biomarkers.

Methods: Proteins were extracted from 8 sera patients with stage I/II thymoma and 9 healthy controls. The levels of serum proteins were detected by data-independent acquisition (DIA) quantitative proteomics techniques, and the differential proteins were identified. The proteomic results were verified by enzyme-linked immunosorbent assay. Additionally, differentially expressed proteins were analyzed using receiver operating characteristic curves (ROC).

Results: There were 80 differentially expressed proteins between the patients with thymoma and the healthy control group, among which 39 were up-regulated and 41 were down-regulated. Differential protein enrichment is involved in environmental information processing, signaling molecules and interactions, and in the body system and the immune system. The analysis of receptor working characteristic curves showed that the areas under the curve of CORO1A, SAA1 and LTA4H were all larger than 0.8, indicating that these proteins had good diagnostic value.

Conclusion: CORO1A, SAA1 and LTA4H may be new biomarkers for early screening of thymoma.

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基于血清蛋白质组学探索早期胸腺瘤的潜在生物标记物

背景：由于胸腺瘤的早期症状不典型，因此早期诊断仍很困难：由于胸腺瘤的早期症状不典型，因此早期诊断仍很困难：本研究旨在通过蛋白质组学方法分析胸腺瘤早期（I/II期）血清蛋白的变化，并筛选和验证候选生物标志物：从8名I/II期胸腺瘤患者和9名健康对照者的血清中提取蛋白质。方法：从 8 例 I/II 期胸腺瘤患者和 9 例健康对照者的血清中提取蛋白质，采用数据独立获取（DIA）定量蛋白质组学技术检测血清蛋白水平，并鉴定差异蛋白。蛋白质组学结果通过酶联免疫吸附试验进行了验证。此外，还利用接收者操作特征曲线（ROC）对差异表达蛋白进行了分析：结果：胸腺瘤患者与健康对照组之间共有 80 个差异表达蛋白，其中 39 个上调，41 个下调。差异蛋白富集涉及环境信息处理、信号分子和相互作用、机体系统和免疫系统。受体工作特征曲线分析表明，CORO1A、SAA1和LTA4H的曲线下面积均大于0.8，表明这些蛋白质具有良好的诊断价值：结论：CORO1A、SAA1和LTA4H可能是早期筛查胸腺瘤的新生物标记物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Protein and Peptide Letters 生物-生化与分子生物学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Protein & Peptide Letters publishes letters, original research papers, mini-reviews and guest edited issues in all important aspects of protein and peptide research, including structural studies, advances in recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, and drug design. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallization and preliminary structure determination of biologically important proteins are considered only if they include significant new approaches or deal with proteins of immediate importance, and preliminary structure determinations of biologically important proteins. Purely theoretical/review papers should provide new insight into the principles of protein/peptide structure and function. Manuscripts describing computational work should include some experimental data to provide confirmation of the results of calculations. Protein & Peptide Letters focuses on: Structure Studies Advances in Recombinant Expression Drug Design Chemical Synthesis Function Pharmacology Enzymology Conformational Analysis Immunology Biotechnology Protein Engineering Protein Folding Sequencing Molecular Recognition Purification and Analysis