Begoña Carrasco, Rubén Torres, María Moreno-Del Álamo, Cristina Ramos, Silvia Ayora, Juan C Alonso
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引用次数: 0
Abstract
Accurate DNA replication and transcription elongation are crucial for preventing the accumulation of unreplicated DNA and genomic instability. Cells have evolved multiple mechanisms to deal with impaired replication fork progression, challenged by both intrinsic and extrinsic impediments. The bacterium Bacillus subtilis, which adopts multiple forms of differentiation and development, serves as an excellent model system for studying the pathways required to cope with replication stress to preserve genomic stability. This review focuses on the genetics, single molecule choreography, and biochemical properties of the proteins that act to circumvent the replicative arrest allowing the resumption of DNA synthesis. The RecA recombinase, its mediators (RecO, RecR, and RadA/Sms) and modulators (RecF, RecX, RarA, RecU, RecD2, and PcrA), repair licensing (DisA), fork remodelers (RuvAB, RecG, RecD2, RadA/Sms, and PriA), Holliday junction resolvase (RecU), nucleases (RnhC and DinG), and translesion synthesis DNA polymerases (PolY1 and PolY2) are key functions required to overcome a replication stress, provided that the fork does not collapse.
准确的 DNA 复制和转录延伸对于防止未复制 DNA 的积累和基因组的不稳定性至关重要。细胞进化出了多种机制,以应对复制叉进展受阻的问题,这些机制同时受到内在和外在障碍的挑战。枯草杆菌采用多种分化和发育形式,是研究应对复制压力以保持基因组稳定性所需途径的极佳模式系统。这篇综述将重点介绍规避复制停滞、恢复 DNA 合成的蛋白质的遗传学、单分子编排和生化特性。其中包括 RecA 重组酶、其介导因子(RecO、RecR、RadA/Sms)和调节因子(RecF、RecX、RarA、RecU、RecD2、PcrA)、修复许可因子(DisA)、叉重塑因子(RuvAB、RecG、RecD2、RadA/Sms、PriA)、霍利迪连接分解酶(RecU)、核酸酶(RnhC、DinG)和转子合成 DNA 聚合酶(PolY1 和 PolY2)是克服复制应激所需的关键功能,前提是叉没有崩溃。
期刊介绍:
Title: FEMS Microbiology Reviews
Journal Focus:
Publishes reviews covering all aspects of microbiology not recently surveyed
Reviews topics of current interest
Provides comprehensive, critical, and authoritative coverage
Offers new perspectives and critical, detailed discussions of significant trends
May contain speculative and selective elements
Aimed at both specialists and general readers
Reviews should be framed within the context of general microbiology and biology
Submission Criteria:
Manuscripts should not be unevaluated compilations of literature
Lectures delivered at symposia must review the related field to be acceptable