PDAT1 genome editing reduces hydroxy fatty acid production in transgenic Arabidopsis.

IF 2.9 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
BMB Reports Pub Date : 2024-02-01
Mid-Eum Park, Hyun Uk Kim
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引用次数: 0

Abstract

The fatty acids content of castor (Ricinus communis L.) seed oil is 80-90% ricinoleic acid, which is a hydroxy fatty acid (HFA). The structures and functional groups of HFAs are different from those of common fatty acids and are useful for various industrial applications. However, castor seeds contain the toxin ricin and an allergenic protein, which limit their cultivation. Accordingly, many researchers are conducting studies to enhance the production of HFAs in Arabidopsis thaliana, a model plant for oil crops. Oleate 12-hydroxylase from castor (RcFAH12), which synthesizes HFA (18:1-OH), was transformed into an Arabidopsis fae1 mutant, resulting in the CL37 line producing a maximum of 17% HFA content. In addition, castor phospholipid:diacylglycerol acyltransferase 1-2 (RcPDAT1-2), which catalyzes the production of triacylglycerol by transferring HFA from phosphatidylcholine to diacylglycerol, was transformed into the CL37 line to develop a P327 line that produces 25% HFA. In this study, we investigated changes in HFA content when endogenous Arabidopsis PDAT1 (AtPDAT1) of the P327 line was edited using the CRISPR/Cas9 technique. The successful mutation resulted in three independent lines with different mutation patterns, which were transmitted until the T4 generation. Fatty acid analysis of the seeds showed that HFA content decreased in all three mutant lines. These findings indicate that AtPDAT1 as well as RcPDAT1-2 in the P327 line are involved in transferring and increasing HFAs to triacylglycerol. [BMB Reports 2024; 57(2): 86-91].

PDAT1 基因组编辑减少了转基因拟南芥中羟基脂肪酸的产生。
蓖麻籽油(Ricinus communis L.)的脂肪酸含量为 80-90%,属于羟基脂肪酸(HFA)。羟基脂肪酸的结构和官能团与普通脂肪酸不同,可用于各种工业用途。然而,蓖麻籽含有毒素蓖麻毒素和一种致敏蛋白质,这限制了蓖麻籽的种植。因此,许多研究人员正在拟南芥(油料作物的模式植物)中开展研究,以提高氢脂肪酸的产量。将合成 HFA(18:1-OH)的蓖麻油酸 12-羟化酶(RcFAH12)转化到拟南芥 fae1 突变体中,结果 CL37 株系的 HFA 含量最高达到 17%。此外,我们还将蓖麻磷脂:二酰甘油酰基转移酶 1-2 (RcPDAT1-2)转化到 CL37 株系中,培育出能产生 25% HFA 的 P327 株系。在本研究中,我们研究了利用 CRISPR/Cas9 技术编辑 P327 株系的内源拟南芥 PDAT1(AtPDAT1)时 HFA 含量的变化。成功的突变产生了三个具有不同突变模式的独立品系,这些品系一直传至 T4 代。种子的脂肪酸分析表明,三个突变品系的 HFA 含量都有所下降。这些发现表明,P327品系中的AtPDAT1和RcPDAT1-2参与了氢氟烷烃向三酰甘油的转移和增加。
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来源期刊
BMB Reports
BMB Reports 生物-生化与分子生物学
CiteScore
5.10
自引率
7.90%
发文量
141
审稿时长
1 months
期刊介绍: The BMB Reports (BMB Rep, established in 1968) is published at the end of every month by Korean Society for Biochemistry and Molecular Biology. Copyright is reserved by the Society. The journal publishes short articles and mini reviews. We expect that the BMB Reports will deliver the new scientific findings and knowledge to our readers in fast and timely manner.
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