Functional characterization of Clonorchis sinensis choline transporter.

0 PARASITOLOGY
Parasites, hosts and diseases Pub Date : 2023-11-01 Epub Date: 2023-11-28 DOI:10.3347/PHD.23082
Jeong Yeon Won, Johnsy Mary Louis, Eui Sun Roh, Seok Ho Cha, Jin-Hee Han
{"title":"Functional characterization of Clonorchis sinensis choline transporter.","authors":"Jeong Yeon Won, Johnsy Mary Louis, Eui Sun Roh, Seok Ho Cha, Jin-Hee Han","doi":"10.3347/PHD.23082","DOIUrl":null,"url":null,"abstract":"<p><p>Clonorchis sinensis is commonly found in East Asian countries. Clonorchiasis is prevalent in these countries and can lead to various clinical symptoms. In this study, we used overlap extension polymerase chain reaction (PCR) and the Xenopus laevis oocyte expression system to isolate a cDNA encoding the choline transporter of C. sinensis (CsChT). We subsequently characterized recombinant CsChT. Expression of CsChT in X. laevis oocytes enabled efficient transport of radiolabeled choline, with no detectable uptake of arginine, α-ketoglutarate, p-aminohippurate, taurocholate, and estrone sulfate. Influx and efflux experiments showed that CsChT-mediated choline uptake was time- and sodium-dependent, with no exchange properties. Concentration-dependent analyses of revealed saturable kinetics consistent with the Michaelis-Menten equation, while nonlinear regression analyses revealed a Km value of 8.3 μM and a Vmax of 61.0 pmol/oocyte/h. These findings contribute to widen our understanding of CsChT transport properties and the cascade of choline metabolisms within C. sinensis.</p>","PeriodicalId":74397,"journal":{"name":"Parasites, hosts and diseases","volume":"61 4","pages":"428-438"},"PeriodicalIF":0.0000,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10693965/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Parasites, hosts and diseases","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3347/PHD.23082","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/11/28 0:00:00","PubModel":"Epub","JCR":"0","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Clonorchis sinensis is commonly found in East Asian countries. Clonorchiasis is prevalent in these countries and can lead to various clinical symptoms. In this study, we used overlap extension polymerase chain reaction (PCR) and the Xenopus laevis oocyte expression system to isolate a cDNA encoding the choline transporter of C. sinensis (CsChT). We subsequently characterized recombinant CsChT. Expression of CsChT in X. laevis oocytes enabled efficient transport of radiolabeled choline, with no detectable uptake of arginine, α-ketoglutarate, p-aminohippurate, taurocholate, and estrone sulfate. Influx and efflux experiments showed that CsChT-mediated choline uptake was time- and sodium-dependent, with no exchange properties. Concentration-dependent analyses of revealed saturable kinetics consistent with the Michaelis-Menten equation, while nonlinear regression analyses revealed a Km value of 8.3 μM and a Vmax of 61.0 pmol/oocyte/h. These findings contribute to widen our understanding of CsChT transport properties and the cascade of choline metabolisms within C. sinensis.

华支睾吸虫胆碱转运体的功能表征。
华支睾吸虫病常见于东亚国家。支睾吸虫病在这些国家很流行,可导致各种临床症状。本研究利用重叠延伸聚合酶链反应(PCR)和非洲爪蟾卵母细胞表达系统分离出中华爪蟾胆碱转运蛋白(CsChT) cDNA。我们随后对重组CsChT进行了表征。CsChT在黄颡鱼卵母细胞中的表达能够有效地运输放射性标记的胆碱,而没有检测到精氨酸、α-酮戊二酸、对氨基马粪酸、牛磺胆酸盐和硫酸雌酮的摄取。内流和外排实验表明,cscht介导的胆碱摄取是时间和钠依赖的,没有交换性质。浓度依赖性分析显示饱和动力学符合Michaelis-Menten方程,非线性回归分析显示Km值为8.3 μM, Vmax为61.0 pmol/卵母细胞/h。这些发现有助于拓宽我们对中华绒螯虾胆碱转运特性和胆碱代谢级联的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
2.70
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信