Antimicrobial Efficacy of Allium cepa and Zingiber officinale Against the Milk-Borne Pathogen Listeria monocytogenes.

IF 3.3 4区 生物学 Q2 MICROBIOLOGY
Journal of Microbiology Pub Date : 2023-11-01 Epub Date: 2023-12-04 DOI:10.1007/s12275-023-00086-w
Abirami Arasu, Nagaram Prabha, Durga Devi, Praveen Kumar Issac, Khaloud Mohammed Alarjani, Dunia A Al Farraj, Reem A Aljeidi, Dina S Hussein, Magesh Mohan, Jehad Zuhair Tayyeb, Ajay Guru, Jesu Arockiaraj
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Abstract

Listeria monocytogenes is an important food-borne pathogen that causes listeriosis and has a high case fatality rate despite its low incidence. Medicinal plants and their secondary metabolites have been identified as potential antibacterial substances, serving as replacements for synthetic chemical compounds. The present studies emphasize two significant medicinal plants, Allium cepa and Zingiber officinale, and their efficacy against L. monocytogenes. Firstly, a bacterial isolate was obtained from milk and identified through morphology and biochemical reactions. The species of the isolate were further confirmed through 16S rRNA analysis. Furthermore, polar solvents such as methanol and ethanol were used for the extraction of secondary metabolites from A. cepa and Z. officinale. Crude phytochemical components were identified using phytochemical tests, FTIR, and GC-MS. Moreover, the antibacterial activity of the crude extract and its various concentrations were tested against L. monocytogenes. Among all, A. cepa in methanolic extracts showed significant inhibitory activity. Since, the A. cepa for methanolic crude extract was used to perform autography to assess its bactericidal activity. Subsequently, molecular docking was performed to determine the specific compound inhibition. The docking results revealed that four compounds displayed strong binding affinity with the virulence factor Listeriolysin-O of L. monocytogenes. Based on the above results, it can be concluded that the medicinal plant A. cepa has potential antibacterial effects against L. monocytogenes, particularly targeting its virulence.

Abstract Image

葱、姜对乳源性单核细胞增生李斯特菌的抑菌效果研究。
单核细胞增生李斯特菌是引起李斯特菌病的重要食源性病原体,发病率低,病死率高。药用植物及其次生代谢物已被确定为潜在的抗菌物质,可作为合成化合物的替代品。本文着重研究了两种重要的药用植物——葱和生姜对单核细胞增生乳杆菌的抑制作用。首先,从牛奶中分离得到一株细菌,并通过形态学和生化反应对其进行鉴定。通过16S rRNA分析进一步确定了分离物的种类。此外,还采用甲醇和乙醇等极性溶剂提取了香薷和山茱萸的次生代谢产物。采用植物化学试验、FTIR和GC-MS鉴定了粗植物化学成分。此外,还研究了不同浓度的粗提物对单核增生乳杆菌的抗菌活性。其中,A. cepa甲醇提取物具有显著的抑制活性。因此,本研究采用乙酸乙酯甲醇粗提物进行了杀菌活性测定。随后,进行分子对接以确定具体的化合物抑制作用。对接结果显示,4个化合物与单核增生乳杆菌的毒力因子李斯特菌溶素- o具有较强的结合亲和力。综上所述,药用植物cepa对单核增生乳杆菌具有潜在的抑菌作用,特别是针对其毒力。
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来源期刊
Journal of Microbiology
Journal of Microbiology 生物-微生物学
CiteScore
5.70
自引率
3.30%
发文量
0
审稿时长
3 months
期刊介绍: Publishes papers that deal with research on microorganisms, including archaea, bacteria, yeasts, fungi, microalgae, protozoa, and simple eukaryotic microorganisms. Topics considered for publication include Microbial Systematics, Evolutionary Microbiology, Microbial Ecology, Environmental Microbiology, Microbial Genetics, Genomics, Molecular Biology, Microbial Physiology, Biochemistry, Microbial Pathogenesis, Host-Microbe Interaction, Systems Microbiology, Synthetic Microbiology, Bioinformatics and Virology. Manuscripts dealing with simple identification of microorganism(s), cloning of a known gene and its expression in a microbial host, and clinical statistics will not be considered for publication by JM.
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