Effect of cationic dendrimer on membrane mimetic systems in the form of monolayer and bilayer

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry and Physics of Lipids Pub Date : 2023-11-30 DOI:10.1016/j.chemphyslip.2023.105364

Biplab Roy , Pritam Guha , Chien-Hsiang Chang , Prasant Nahak , Gourab Karmakar , Alexey G. Bykov , Alexander V. Akentiev , Boris A. Noskov , Anuttam Patra , Kunal Dutta , Chandradipa Ghosh , Amiya Kumar Panda

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Abstract

Interactions between a zwitterionic phospholipid, 1, 2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) and four anionic phospholipids dihexadecyl phosphate (DHP), 1, 2-dimyristoyl-sn-glycero-3-phosphoglycerol (DMPG), 1, 2-dipalmitoyl-sn-glycero-3-phosphate (DPP) and 1, 2-dipalmitoyl-sn-glycero-3-phospho ethanol (DPPEth) in combination with an additional amount of 30 mol% cholesterol were separately investigated at air-buffer interface through surface pressure (π) - area (A) measurements. π-A isotherm derived parameters revealed maximum negative deviation from ideality for the mixtures comprising 30 mol% anionic lipids. Besides the film functionality, structural changes of the monomolecular films at different surface pressures in the absence and presence of polyamidoamine (PAMAM, generation 4), a cationic dendrimer, were visualised through Brewster angle microscopy and fluorescence microscopic studies. Fluidity/rigidity of monolayers were assessed by surface dilatational rheology studies. Effect of PAMAM on the formation of adsorbed monolayer, due to bilayer disintegration of liposomes (DPPC:anionic lipids= 7:3 M/M, and 30 mol% cholesterol) were monitored by surface pressure (π) - time (t) isotherms. Bilayer disintegration kinetics were dependent on lipid head group and chain length, besides dendrimer concentration. Such studies are considered to be an in vitro cell membrane model where the alteration of molecular orientation play important roles in understanding the nature of interaction between the dendrimer and cell membrane. Liposome-dendrimer aggregates were nontoxic to breast cancer cell line as well as in doxorubicin treated MDA-MB-468 cell line suggesting their potential as drug delivery systems.

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阳离子树状大分子对单层和双层膜模拟体系的影响。

通过测量表面压力(π) -面积(a)，分别研究了两性离子磷脂、1,2 -二棕榈酰基- n-甘油-3-磷脂酰胆碱(DPPC)和四种阴离子磷脂二十六进基磷酸(DHP)、1,2 -二棕榈酰基- n-甘油-3-磷酸(DMPG)、1,2 -二棕榈酰基- n-甘油-3-磷酸(DPP)和1,2 -二棕榈酰基- n-甘油-3-磷酸乙醇(dpeth)与附加量30摩尔%胆固醇在空气缓冲界面上的相互作用。π-A等温线推导出的参数显示，对于含有30mol %阴离子脂质的混合物，π-A等温线与理想状态的最大负偏差。除了膜的功能外，通过布鲁斯特角显微镜和荧光显微镜研究，在不存在和存在阳离子树状大分子聚酰胺胺(PAMAM，第4代)的情况下，单分子膜在不同表面压力下的结构变化。通过表面膨胀流变学研究评估了单层膜的流动性/刚性。通过表面压力(π) -时间(t)等温线监测PAMAM对脂质体(DPPC:阴离子脂质体= 7:3M/M, 30mol %胆固醇)双层崩解形成吸附单层的影响。除树突分子浓度外，双分子层分解动力学还与脂质头基团和链长有关。这些研究被认为是一种体外细胞膜模型，其中分子取向的改变对理解树突与细胞膜之间相互作用的性质起着重要作用。脂质体-树状大分子聚集体对乳腺癌细胞系以及阿霉素处理的MDA-MB-468细胞系均无毒，提示其作为药物传递系统的潜力。

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来源期刊

Chemistry and Physics of Lipids 生物-生化与分子生物学

CiteScore

7.60

自引率

2.90%

发文量

审稿时长

40 days

期刊介绍： Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.