NSUN6 Regulates NM23-H1 Expression in an m5C Manner to Affect Epithelial-Mesenchymal Transition in Lung Cancer.

IF 2.9 3区医学 Q1 MEDICINE, GENERAL & INTERNAL

Medical Principles and Practice Pub Date : 2024-01-01 Epub Date: 2023-11-29 DOI:10.1159/000535479

Zhansheng Lu, Bo Liu, Demiao Kong, Xiaojiang Zhou, Dengke Pei, Di Liu

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引用次数: 0

Abstract

Purpose: The expression and regulatory mechanism of NSUN6 in lung cancer are still unclear. Our study explored whether NSUN6 mediates progression of lung cancer by affecting NM23-H1 expression in an m5C-dependent manner.

Methods: qRT-PCR, CCK-8, colony formation, transwell, and Western blot analysis were employed to probe the impact of NSUN6 on lung cancer cell proliferation, migration, and epithelial-mesenchymal transition (EMT). RMVar database was utilized to forecast the downstream genes of NSUN6. The mode of interaction between NSUN6 and NM23-H1 was determined by dot blot, luciferase assay, m5C RIP, and cell function assays. The effect of NSUN6 expression on tumor growth was verified in vivo.

Results: Expression of NSUN6 was reduced in lung cancer cells, and over-expression of NSUN6 restricted the proliferation of lung cancer cells, migration, and EMT. NSUN6 regulated NM23-H1 expression by modifying the 3'-UTR of NM23-H1 mRNA through m5C and inhibited lung cancer cell proliferation, migration, and EMT. In vivo experiments also showed that over-expression of NSUN6 inhibited the occurrence of lung cancer.

Conclusion: NSUN6 regulates NM23-H1 expression in an m5C-dependent manner to affect EMT in lung cancer. Thus, NSUN6 may be considered as a potential therapeutic target for lung cancer.

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NSUN6以m5C方式调控NM23-H1表达，影响肺癌上皮-间质转化。

目的:NSUN6在肺癌中的表达及调控机制尚不清楚。我们的研究探讨了NSUN6是否通过m5c依赖的方式影响NM23-H1的表达来介导肺癌的进展。方法:采用qRT-PCR、CCK-8、集落形成、transwell和western blot分析NSUN6对肺癌细胞增殖、迁移和上皮间质转化(EMT)的影响。利用RMVar数据库对NSUN6下游基因进行预测。采用点印迹法、荧光素酶法、m5C RIP法和细胞功能法确定NSUN6与NM23-H1的相互作用模式。体内实验验证了NSUN6表达对肿瘤生长的影响。结果:NSUN6在肺癌细胞中的表达降低，过表达抑制了肺癌细胞的增殖、迁移和EMT。NSUN6通过m5C修饰NM23-H1 mRNA的3′-UTR，调控NM23-H1的表达，抑制肺癌细胞的增殖、迁移和EMT。体内实验也表明，过表达NSUN6可抑制肺癌的发生。结论:NSUN6以m5c依赖的方式调控NM23-H1的表达，影响肺癌的EMT。因此，NSUN6可能被认为是肺癌的潜在治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Medical Principles and Practice 医学-医学：内科

CiteScore

6.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Medical Principles and Practice'', as the journal of the Health Sciences Centre, Kuwait University, aims to be a publication of international repute that will be a medium for dissemination and exchange of scientific knowledge in the health sciences.