{"title":"Analysis of five cases showing false-high Hemoglobin A1c due to reduced catalase activity.","authors":"Kenji Hara, Atsushi Ujiie, Shiori Suzuki, Takenori Okumura, Mio Kubo, Hiroyuki Shinozaki, Mototaka Yamauchi, Takafumi Tsuchiya, Kohzo Takebayashi, Nobuyuki Shimozawa, Masafumi Koga, Koshi Hashimoto","doi":"10.1507/endocrj.EJ23-0212","DOIUrl":null,"url":null,"abstract":"<p><p>We encountered five cases that exhibited false-high Hemoglobin A1c (HbA1c) levels when samples were examined using the enzyme-based NORUDIA N HbA1c kit. HbA1c levels were higher than those obtained using other methods, such as HPLC, immune-based methods, and other enzyme-based kits. This kit produced inaccurate results for HbA1c when residual peroxides were present in samples. The addition of peroxidase solution restored false-high HbA1c levels in the five cases, indicating that reduced catalase activity was responsible for these values because catalase eliminates peroxide. Catalase activity and gene mutations were examined in the five cases and an immunohistological analysis was performed to assess the expression of catalase. Cases #1 and 2 were diagnosed as acatalasemia and cases #3, 4, and 5 as hypocatalasemia based on compound heterozygous SNP and heterozygous splicing mutations in the catalase gene. Therefore, impaired catalase activity was responsible for false-high HbA1c levels measured by the NORUDIA N HbA1c kit.</p>","PeriodicalId":11631,"journal":{"name":"Endocrine journal","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Endocrine journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1507/endocrj.EJ23-0212","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/11/28 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 0
Abstract
We encountered five cases that exhibited false-high Hemoglobin A1c (HbA1c) levels when samples were examined using the enzyme-based NORUDIA N HbA1c kit. HbA1c levels were higher than those obtained using other methods, such as HPLC, immune-based methods, and other enzyme-based kits. This kit produced inaccurate results for HbA1c when residual peroxides were present in samples. The addition of peroxidase solution restored false-high HbA1c levels in the five cases, indicating that reduced catalase activity was responsible for these values because catalase eliminates peroxide. Catalase activity and gene mutations were examined in the five cases and an immunohistological analysis was performed to assess the expression of catalase. Cases #1 and 2 were diagnosed as acatalasemia and cases #3, 4, and 5 as hypocatalasemia based on compound heterozygous SNP and heterozygous splicing mutations in the catalase gene. Therefore, impaired catalase activity was responsible for false-high HbA1c levels measured by the NORUDIA N HbA1c kit.
当使用基于酶的NORUDIA N HbA1c试剂盒检测样品时,我们遇到了5例表现出假高血红蛋白A1c (HbA1c)水平的病例。HbA1c水平高于其他方法,如HPLC法、免疫法和其他酶基试剂盒。当样品中存在残余过氧化物时,该试剂盒产生的HbA1c结果不准确。添加过氧化物酶溶液恢复了5例患者的假高HbA1c水平,表明过氧化氢酶活性降低是造成这些值的原因,因为过氧化氢酶消除了过氧化物。检测5例患者的过氧化氢酶活性和基因突变,并进行免疫组织学分析以评估过氧化氢酶的表达。根据过氧化氢酶基因的复合杂合SNP和杂合剪接突变,病例#1和2诊断为失氢酶血症,病例#3、4和5诊断为低过氧化氢血症。因此,过氧化氢酶活性受损是NORUDIA N HbA1c试剂盒测量的假高HbA1c水平的原因。
期刊介绍:
Endocrine Journal is an open access, peer-reviewed online journal with a long history. This journal publishes peer-reviewed research articles in multifaceted fields of basic, translational and clinical endocrinology. Endocrine Journal provides a chance to exchange your ideas, concepts and scientific observations in any area of recent endocrinology. Manuscripts may be submitted as Original Articles, Notes, Rapid Communications or Review Articles. We have a rapid reviewing and editorial decision system and pay a special attention to our quick, truly scientific and frequently-citable publication. Please go through the link for author guideline.