In vitro and in vivo effect of oxytetracycline on sperm parameters in breeding rooster.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-09-01

L Mohammedi, A Messai, L Touazi, M Iguer-Ouada

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引用次数: 0

Abstract

Background: Some antimicrobials could adversely affect sperm quality during sperm cryopreservation and antibiotic treatment with subsequent effects on fertility outputs. To our knowledge, no similar studies have been conducted on breeding roosters, especially for oxytetracycline (OTC).

Objective: To investigate both in vitro and in vivo impact of oxytetracycline on sperm parameters in breeding roosters.

Methods: Sperm motility parameters were objectively analyzed using the CASA system including total motility (TM %), progressive motility (PM %), all sperm velocities, the sperm count, and cell viability during 9 days of in vivo treatment. In the in vitro investigation, the pooled sperm was diluted and divided into a control aliquot (diluted in 0.9% NaCl) and treated samples. Motility parameters were assessed after 0, 1, 2, 3, 4, 5, and 6 hours of storage at 37ºC. In the in vivo study, 1 g per L of OTC was administrated to five individuals for nine consecutive days. Fresh semen samples were analyzed at T0 (before treatment) and after 6 (T6) and 9 days (T9) of treatment.

Results: OTC caused significant impairment of sperm quality in vivo. A drastic reduction in sperm concentration, viability, TM, PM, and all kinematic parameters was observed after 6 days of treatment. However, at day 9 sperm quality had improved to be nearly similar to T0. In vitro, OTC induced similar sperm impairment on all sperm motility parameters.

Conclusion: Oxytetracycline exhibited negative effects on rooster sperm both in vivo and in vitro and appears consequently not suitable in cryopreservation extenders. Doi.org/10.54680/fr23510110412.

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土霉素对种鸡精子参数的体内外影响。

背景:在精子冷冻保存和抗生素治疗过程中，一些抗菌剂可能会对精子质量产生不利影响，从而影响生育产出。据我们所知，还没有对饲养公鸡进行过类似的研究，特别是对土霉素(OTC)。目的:研究土霉素在体内外对种鸡精子参数的影响。方法:采用CASA系统客观分析精子运动参数，包括总运动力(TM %)、进展运动力(PM %)、所有精子速度、精子数量和细胞活力。在体外研究中，将混合的精子稀释后分为对照(用0.9% NaCl稀释)和处理过的样品。在37ºC下储存0、1、2、3、4、5和6小时后评估运动参数。在体内研究中，5个人连续9天服用1 g / L的OTC。在治疗前(T0)、治疗后6天(T6)和9天(T9)对新鲜精液样本进行分析。结果:OTC在体内对精子质量有明显损害。治疗6天后，观察到精子浓度、活力、TM、PM和所有运动学参数急剧下降。然而，在第9天，精子质量已经改善到几乎与第0天相似。在体外，OTC对精子的所有运动参数都有类似的损伤。结论:土霉素在体内和体外对公鸡精子均有不良影响，不宜作为低温保存的延长剂。Doi.org/10.54680/fr23510110412。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.