Coronin-1 promotes directional cell rearrangement in Drosophila wing epithelium.

IF 2 4区 生物学 Q4 CELL BIOLOGY
Cell structure and function Pub Date : 2023-12-20 Epub Date: 2023-11-30 DOI:10.1247/csf.23049
Keisuke Ikawa, Souta Hiro, Shu Kondo, Shizue Ohsawa, Kaoru Sugimura
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引用次数: 0

Abstract

Directional cell rearrangement is a critical process underlying correct tissue deformation during morphogenesis. Although the involvement of F-actin regulation in cell rearrangement has been established, the role and regulation of actin binding proteins (ABPs) in this process are not well understood. In this study, we investigated the function of Coronin-1, a WD-repeat actin-binding protein, in controlling directional cell rearrangement in the Drosophila pupal wing. Transgenic flies expressing Coronin-1-EGFP were generated using CRISPR-Cas9. We observed that Coronin-1 localizes at the reconnecting junction during cell rearrangement, which is dependent on actin interacting protein 1 (AIP1) and cofilin, actin disassemblers and known regulators of wing cell rearrangement. Loss of Coronin-1 function reduces cell rearrangement directionality and hexagonal cell fraction. These results suggest that Coronin-1 promotes directional cell rearrangement via its interaction with AIP1 and cofilin, highlighting the role of ABPs in the complex process of morphogenesis.Key words: morphogenesis, cell rearrangement, actin binding proteins (ABPs).

冠状蛋白-1促进果蝇翅膀上皮细胞定向重排。
定向细胞重排是形态发生过程中组织正确变形的关键过程。虽然已经确定f -肌动蛋白参与细胞重排的调节,但肌动蛋白结合蛋白(ABPs)在这一过程中的作用和调节尚不清楚。在本研究中,我们研究了一种WD-repeat肌动蛋白结合蛋白Coronin-1在果蝇蛹中控制细胞定向重排的功能。利用CRISPR-Cas9技术生成了表达Coronin-1-EGFP的转基因果蝇。我们观察到,在细胞重排过程中,冠状蛋白1定位于重连接节点,这依赖于肌动蛋白相互作用蛋白1 (AIP1)和cofilin,肌动蛋白拆解子和已知的翅膀细胞重排调节因子。冠状蛋白-1功能的丧失降低了细胞重排方向性和六边形细胞比例。这些结果表明,Coronin-1通过与AIP1和cofilin的相互作用促进定向细胞重排,突出了ABPs在复杂的形态发生过程中的作用。关键词:形态发生,细胞重排,肌动蛋白结合蛋白(ABPs)
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来源期刊
Cell structure and function
Cell structure and function 生物-细胞生物学
CiteScore
2.50
自引率
0.00%
发文量
6
审稿时长
>12 weeks
期刊介绍: Cell Structure and Function is a fully peer-reviewed, fully Open Access journal. As the official English-language journal of the Japan Society for Cell Biology, it is published continuously online and biannually in print. Cell Structure and Function publishes important, original contributions in all areas of molecular and cell biology. The journal welcomes the submission of manuscripts on research areas such as the cell nucleus, chromosomes, and gene expression; the cytoskeleton and cell motility; cell adhesion and the extracellular matrix; cell growth, differentiation and death; signal transduction; the protein life cycle; membrane traffic; and organelles.
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