Systematic arrangement within the family Clitocybaceae (Tricholomatineae, Agaricales): phylogenetic and phylogenomic evidence, morphological data and muscarine-producing innovation

IF 24.5 1区 生物学 Q1 MYCOLOGY
Zheng-Mi He, Zuo-Hong Chen, Tolgor Bau, Geng-Shen Wang, Zhu L. Yang
{"title":"Systematic arrangement within the family Clitocybaceae (Tricholomatineae, Agaricales): phylogenetic and phylogenomic evidence, morphological data and muscarine-producing innovation","authors":"Zheng-Mi He, Zuo-Hong Chen, Tolgor Bau, Geng-Shen Wang, Zhu L. Yang","doi":"10.1007/s13225-023-00527-2","DOIUrl":null,"url":null,"abstract":"<p>The Clitocybaceae is a recently established family. Currently, the infrafamilial divisions and relationships within the family are vague due to limited sampling and genes employed for phylogenetic analysis. Some mushrooms of the family contain the neurotoxic muscarine, which has caused many severe and even deadly poisonings worldwide. However, the taxonomic distribution and evolution of the toxin within the family is largely unknown. In this study, phylogenetic analyses based on nucleotide sequences of ITS and of six molecular loci (ITS, LSU, <i>TEF1</i>, <i>RPB1</i>, <i>RPB2</i> and <i>ATP6</i>), plus a phylogenomic analysis based on 485 single-copy orthologous genes, were performed to reconstruct the framework of Clitocybaceae. BEAST analysis was used to estimate the divergence times within the family. Additionally, biochemical analysis for muscarine was conducted of 32 representative species. Based on these analyses, an updated classification of Clitocybaceae into six genera (<i>Clitocybe</i>, <i>Collybia</i>, <i>Dendrocollybia</i>, <i>Lepista</i>, <i>Pseudolyophyllum</i>, and <i>Singerocybe</i>) is proposed. The genus <i>Collybia</i> is emended to accommodate four subgenera (<i>Collybia</i>, <i>Crassicybe</i>, <i>Leucocalocybe</i>, and <i>Macrosporocybe</i>). Seventeen new Chinese species and 15 new combinations are proposed. Keys to the genera of Clitocybaceae and the subgenera of <i>Collybia</i>, as well as to the known species of <i>Clitocybe</i> and <i>Collybia</i> subgen. <i>Collybia</i> in China, are presented. In addition, muscarine was detected in 18 species, and these muscarine-containing species formed a major monophyletic clade within <i>Collybia</i> subgen. <i>Collybia</i>. Finally, our phylogenetic, phylogenomic, chemotaxonomic and molecular dating results indicate that the Clitocybaceae is a natural group estimated to have arisen some 60 million years ago, and in this family, muscarine has evolved only once circa 20 million years ago without later losses.</p>","PeriodicalId":12471,"journal":{"name":"Fungal Diversity","volume":"71 6","pages":""},"PeriodicalIF":24.5000,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fungal Diversity","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s13225-023-00527-2","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The Clitocybaceae is a recently established family. Currently, the infrafamilial divisions and relationships within the family are vague due to limited sampling and genes employed for phylogenetic analysis. Some mushrooms of the family contain the neurotoxic muscarine, which has caused many severe and even deadly poisonings worldwide. However, the taxonomic distribution and evolution of the toxin within the family is largely unknown. In this study, phylogenetic analyses based on nucleotide sequences of ITS and of six molecular loci (ITS, LSU, TEF1, RPB1, RPB2 and ATP6), plus a phylogenomic analysis based on 485 single-copy orthologous genes, were performed to reconstruct the framework of Clitocybaceae. BEAST analysis was used to estimate the divergence times within the family. Additionally, biochemical analysis for muscarine was conducted of 32 representative species. Based on these analyses, an updated classification of Clitocybaceae into six genera (Clitocybe, Collybia, Dendrocollybia, Lepista, Pseudolyophyllum, and Singerocybe) is proposed. The genus Collybia is emended to accommodate four subgenera (Collybia, Crassicybe, Leucocalocybe, and Macrosporocybe). Seventeen new Chinese species and 15 new combinations are proposed. Keys to the genera of Clitocybaceae and the subgenera of Collybia, as well as to the known species of Clitocybe and Collybia subgen. Collybia in China, are presented. In addition, muscarine was detected in 18 species, and these muscarine-containing species formed a major monophyletic clade within Collybia subgen. Collybia. Finally, our phylogenetic, phylogenomic, chemotaxonomic and molecular dating results indicate that the Clitocybaceae is a natural group estimated to have arisen some 60 million years ago, and in this family, muscarine has evolved only once circa 20 million years ago without later losses.

Abstract Image

阴蒂菌科的系统排列:系统发育和系统基因组证据、形态数据和产生毒蕈碱的创新
阴蒂菌科是一个新近成立的科。目前,由于样本和用于系统发育分析的基因有限,家族内的家族内部划分和关系模糊不清。该家族的一些蘑菇含有神经毒性毒蕈碱,在世界范围内引起了许多严重甚至致命的中毒。然而,毒素在该家族中的分类分布和进化在很大程度上是未知的。本研究基于ITS和6个分子位点(ITS、LSU、TEF1、RPB1、RPB2和ATP6)的核苷酸序列,以及基于485个单拷贝同源基因的系统发育分析,重建了clitocybacae的结构框架。使用BEAST分析来估计家族内部的分化时间。并对32种有代表性的毒蕈碱进行了生化分析。在此基础上,提出了一种更新的clitocybacae分类方法:Clitocybe、Collybia、Dendrocollybia、Lepista、Pseudolyophyllum和Singerocybe。Collybia属被修正为容纳四个亚属(Collybia, assicybe, Leucocalocybe和Macrosporocybe)。提出了17个中国新种和15个新组合。cliitocybacae属和Collybia亚属的关键字,以及已知的cliitocybe和Collybia亚属的关键字。在中国的哥伦比亚,都有介绍。此外,在18个物种中检测到毒蕈碱,这些含有毒蕈碱的物种构成了Collybia亚属中一个主要的单系分支。Collybia。最后,我们的系统发育、系统基因组学、化学分类和分子定年结果表明,阴蒂菌科是一个自然的类群,估计大约在6000万年前出现,而在这个科中,muscarine在大约2000万年前只进化过一次,后来没有消失。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Fungal Diversity
Fungal Diversity 生物-真菌学
CiteScore
44.80
自引率
9.90%
发文量
17
审稿时长
6 months
期刊介绍: Fungal Diversity, the official journal of the Kunming Institute of Botany of the Chinese Academy of Sciences, is an international, peer-reviewed journal covering all aspects of mycology. It prioritizes papers on biodiversity, systematic, and molecular phylogeny. While it welcomes novel research and review articles, authors aiming to publish checklists are advised to seek regional journals, and the introduction of new species and genera should generally be supported by molecular data. Published articles undergo peer review and are accessible online first with a permanent DOI, making them citable as the official Version of Record according to NISO RP-8-2008 standards. Any necessary corrections after online publication require the publication of an Erratum.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信