Human placental extract suppresses mast cell activation and induces mast cell apoptosis.

IF 2.6 4区医学 Q2 ALLERGY

Allergy Asthma and Clinical Immunology Pub Date : 2023-11-27 DOI:10.1186/s13223-023-00850-y

Tongqian Wu, Jingjing He, Shirong Yan, Jing Li, Ke Chen, Dingshan Zhang, Mingliang Cheng, Zou Xiang, Yu Fang

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引用次数: 0

Abstract

Background: Human placental extract (HPE) has been documented to facilitate the healing of certain disorders including allergy. However, the effects of HPE on the functionality of mast cells, a critical cell type in allergic diseases, have not been reported.

Methods: To investigate the effects of HPE on the regulation of allergy with respect to the biological functions of mast cells, the mast cell line C57 or HMC-1 cells were treated with HPE followed by the assessment of cell proliferation, apoptosis, activation, chemotaxis and phagocytosis. Mouse peritoneal mast cells were also investigated for their responses to induction of apoptosis by HPE in vivo. Furthermore, the effect of HPE on mast cell degranulation was confirmed using the passive cutaneous anaphylaxis (PCA) assay, an acute allergy model.

Results: HPE was capable of suppressing mast cell proliferation and inducing mast cell apoptosis. Mast cell degranulation in response to compound 48/80- or anti-DNP IgE and DNP-mediated activation was suppressed. In addition, treatment with HPE compromised the production of cytokines by mast cells and cell chemotaxis. These observations were consistent with the dampened passive cutaneous anaphylaxis (PCA) assay following treatment with HPE.

Conclusion: This study revealed a suppressive effect of HPE on overall mast cell activities, suggesting a potential regulatory role of HPE on the alleviation of allergic diseases through mast cells.

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人胎盘提取物抑制肥大细胞活化，诱导肥大细胞凋亡。

背景:人类胎盘提取物(HPE)已被证明可以促进包括过敏在内的某些疾病的愈合。然而，HPE对肥大细胞(过敏性疾病的关键细胞类型)功能的影响尚未报道。方法:研究HPE对肥大细胞生物学功能的调节作用，分别对肥大细胞系C57和HMC-1细胞进行HPE处理，观察细胞增殖、凋亡、活化、趋化和吞噬作用。我们还研究了小鼠腹膜肥大细胞对HPE诱导凋亡的反应。此外，HPE对肥大细胞脱颗粒的影响是通过被动皮肤过敏反应(PCA)试验证实的，这是一种急性过敏模型。结果:HPE具有抑制肥大细胞增殖和诱导肥大细胞凋亡的作用。对复方48/80或抗dnp反应的肥大细胞脱颗粒被抑制，IgE和dnp介导的激活被抑制。此外，HPE治疗损害了肥大细胞产生细胞因子和细胞趋化性。这些观察结果与HPE治疗后的湿性被动皮肤过敏反应(PCA)测定一致。结论:本研究揭示了HPE对肥大细胞整体活性的抑制作用，提示HPE可能通过肥大细胞减轻过敏性疾病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Allergy Asthma and Clinical Immunology ALLERGY-IMMUNOLOGY

CiteScore

4.30

自引率

3.70%

发文量

审稿时长

12 weeks

期刊介绍： Allergy, Asthma & Clinical Immunology (AACI), the official journal of the Canadian Society of Allergy and Clinical Immunology (CSACI), is an open access journal that encompasses all aspects of diagnosis, epidemiology, prevention and treatment of allergic and immunologic disease. By offering a high-visibility forum for new insights and discussions, AACI provides a platform for the dissemination of allergy and clinical immunology research and reviews amongst allergists, pulmonologists, immunologists and other physicians, healthcare workers, medical students and the public worldwide. AACI reports on basic research and clinically applied studies in the following areas and other related topics: asthma and occupational lung disease, rhinoconjunctivitis and rhinosinusitis, drug hypersensitivity, allergic skin diseases, urticaria and angioedema, venom hypersensitivity, anaphylaxis and food allergy, immunotherapy, immune modulators and biologics, immune deficiency and autoimmunity, T cell and B cell functions, regulatory T cells, natural killer cells, mast cell and eosinophil functions, complement abnormalities.