Determining predictive metabolomic biomarkers of meniscal injury in dogs with cranial cruciate ligament rupture

IF 1.7 2区农林科学 Q2 VETERINARY SCIENCES

Journal of Small Animal Practice Pub Date : 2023-11-27 DOI:10.1111/jsap.13688

C. R. Pye, D. C. Green, J. R. Anderson, M. M. Phelan, M. M. Fitzgerald, E. J. Comerford, M. J. Peffers

{"title":"Determining predictive metabolomic biomarkers of meniscal injury in dogs with cranial cruciate ligament rupture","authors":"C. R. Pye, D. C. Green, J. R. Anderson, M. M. Phelan, M. M. Fitzgerald, E. J. Comerford, M. J. Peffers","doi":"10.1111/jsap.13688","DOIUrl":null,"url":null,"abstract":"<div>\n \n <section>\n \n <h3> Objectives</h3>\n \n <p>This study used hydrogen nuclear magnetic resonance spectroscopy for the first time to examine differences in the metabolomic profile of stifle joint synovial fluid from dogs with cranial cruciate ligament rupture with and without meniscal injuries, in order to identify biomarkers of meniscal injury. Identifying a biomarker of meniscal injury could then ultimately be used to design a minimally invasive diagnostic test for meniscal injuries in dogs.</p>\n </section>\n \n <section>\n \n <h3> Materials and Methods</h3>\n \n <p>Stifle joint synovial fluid was collected from dogs undergoing stifle joint surgery or arthrocentesis for lameness investigations. We used multi-variate statistical analysis using principal component analysis and univariate statistical analysis using one-way analysis of variance and analysis of co-variance to identify differences in the metabolomic profile between dogs with cranial cruciate ligament rupture and meniscal injury, cranial cruciate ligament rupture without meniscal injury, and neither cranial cruciate ligament rupture nor meniscal injury, taking into consideration clinical variables.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>A total of 154 samples of canine synovial fluid were included in the study. Sixty-four metabolites were annotated to the hydrogen nuclear magnetic resonance spectroscopy spectra. Six spectral regions were found to be significantly altered (false discovery rate adjusted P-value <0.05) between groups with cranial cruciate ligament rupture with and without meniscal injury, including three attributed to nuclear magnetic resonance mobile lipids [mobile lipid -CH<sub>3</sub> (P=0.016), mobile lipid -n(CH<sub>3</sub>)<sub>3</sub> (P=0.017), mobile unsaturated lipid (P=0.031)].</p>\n </section>\n \n <section>\n \n <h3> Clinical Significance</h3>\n \n <p>We identified an increase in nuclear magnetic resonance mobile lipids in the synovial fluid of dogs with meniscal injury which are of interest as potential biomarkers of meniscal injury.</p>\n </section>\n </div>","PeriodicalId":17062,"journal":{"name":"Journal of Small Animal Practice","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jsap.13688","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Small Animal Practice","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jsap.13688","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives

This study used hydrogen nuclear magnetic resonance spectroscopy for the first time to examine differences in the metabolomic profile of stifle joint synovial fluid from dogs with cranial cruciate ligament rupture with and without meniscal injuries, in order to identify biomarkers of meniscal injury. Identifying a biomarker of meniscal injury could then ultimately be used to design a minimally invasive diagnostic test for meniscal injuries in dogs.

Materials and Methods

Stifle joint synovial fluid was collected from dogs undergoing stifle joint surgery or arthrocentesis for lameness investigations. We used multi-variate statistical analysis using principal component analysis and univariate statistical analysis using one-way analysis of variance and analysis of co-variance to identify differences in the metabolomic profile between dogs with cranial cruciate ligament rupture and meniscal injury, cranial cruciate ligament rupture without meniscal injury, and neither cranial cruciate ligament rupture nor meniscal injury, taking into consideration clinical variables.

Results

A total of 154 samples of canine synovial fluid were included in the study. Sixty-four metabolites were annotated to the hydrogen nuclear magnetic resonance spectroscopy spectra. Six spectral regions were found to be significantly altered (false discovery rate adjusted P-value <0.05) between groups with cranial cruciate ligament rupture with and without meniscal injury, including three attributed to nuclear magnetic resonance mobile lipids [mobile lipid -CH₃ (P=0.016), mobile lipid -n(CH₃)₃ (P=0.017), mobile unsaturated lipid (P=0.031)].

Clinical Significance

We identified an increase in nuclear magnetic resonance mobile lipids in the synovial fluid of dogs with meniscal injury which are of interest as potential biomarkers of meniscal injury.

Abstract Image

查看原文本刊更多论文

确定颅交叉韧带破裂犬半月板损伤的预测性代谢组学生物标志物。

目的:本研究首次使用氢核磁共振波谱技术，检测有半月板损伤和没有半月板损伤的颅十字韧带破裂犬的膝关节滑液代谢组学特征的差异，以确定半月板损伤的生物标志物。识别半月板损伤的生物标志物最终可以用于设计狗半月板损伤的微创诊断测试。材料和方法:从接受膝关节手术或关节置换术的狗身上收集膝关节滑液进行跛行调查。在考虑临床变量的情况下，采用主成分分析的多因素统计分析和单因素方差分析的单因素统计分析，确定颅交叉韧带断裂伴半月板损伤、颅交叉韧带断裂伴半月板损伤、颅交叉韧带断裂伴半月板损伤和无颅交叉韧带断裂伴半月板损伤犬的代谢组学差异。结果:154份犬滑液样本被纳入研究。对64种代谢物进行了氢核磁共振谱标注。6个光谱区被发现显著改变(假发现率调整P值3 (P=0.016)、流动脂质-n(CH3)3 (P=0.017)、流动不饱和脂质(P=0.031))。临床意义:我们在半月板损伤犬的滑液中发现了核磁共振移动脂质的增加，这是半月板损伤的潜在生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Small Animal Practice 农林科学-兽医学

CiteScore

3.30

自引率

6.20%

发文量

117

审稿时长

12-24 weeks

期刊介绍： Journal of Small Animal Practice (JSAP) is a monthly peer-reviewed publication integrating clinical research papers and case reports from international sources, covering all aspects of medicine and surgery relating to dogs, cats and other small animals. These papers facilitate the dissemination and implementation of new ideas and techniques relating to clinical veterinary practice, with the ultimate aim of promoting best practice. JSAP publishes high quality original articles, as well as other scientific and educational information. New developments are placed in perspective, encompassing new concepts and peer commentary. The target audience is veterinarians primarily engaged in the practise of small animal medicine and surgery. In addition to original articles, JSAP will publish invited editorials (relating to a manuscript in the same issue or a topic of current interest), review articles, which provide in-depth discussion of important clinical issues, and other scientific and educational information from around the world. The final decision on publication of a manuscript rests with the Editorial Board and ultimately with the Editor. All papers, regardless of type, represent the opinion of the authors and not necessarily that of the Editor, the Association or the Publisher. The Journal of Small Animal Practice is published on behalf of the British Small Animal Veterinary Association and is also the official scientific journal of the World Small Animal Veterinary Association