Performance evaluation of sperm concentration, motility, and morphological analysis for GSA-810 series of sperm quality analysis system.

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis Pub Date : 2023-12-01 Epub Date: 2023-11-27 DOI:10.1002/jcla.24986

Yan-Mei Ge, Jin-Chun Lu, Shan-Shan Tang, Yuan-Hua Xu, Yuan-Jiao Liang

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Abstract

Background: The performance evaluation of each computer-assisted sperm analysis (CASA) system may provide a basis for the interpretation of clinical results and further improvement of the CASA system.

Methods: The accuracy of the GSA-810 CASA system was evaluated by detecting latex bead quality control products. The precision of sperm concentration, morphology, and percentages of progressively motile sperm (PR) were evaluated by coefficient of variation (CV). Three samples with sperm concentration of about 100 × 10⁶ /mL were diluted to evaluate the linear range.

Results: The detection values of latex beads were within the range of target values. The CVs of sperm concentration and PR were significantly and negatively correlated with sperm concentration (r = -0.561, p = 0.001) and PR value (r = -0.621, p < 0.001), respectively. The R² values of the linear range of sperm concentration were ≥0.99. There was no significant difference in sperm motility and PR within 1-10 min at 36.5°C ± 0.5°C. The coincidence rates of sperm morphology and sperm head morphology for 36 semen samples analyzed by the GSA-810 system and manual method were 99.40% and 99.67%, respectively. The CVs of the percentage of sperm with abnormal morphology and percentage of sperm with abnormal head morphology were less than 5%.

Conclusion: The GSA-810 system can accurately analyze normal semen samples, but the repeatability of the results is poor for oligozoospermia and asthenozoospermia samples. The future CASA system for analyzing sperm morphology should focus on recognizing the middle and tail segments of a spermatozoon.

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GSA-810系列精子质量分析系统的精子浓度、活力及形态分析性能评价。

背景:对各计算机辅助精子分析(CASA)系统的性能进行评价，可为临床结果的解释和进一步完善CASA系统提供依据。方法:通过检测乳胶珠质控品，对GSA-810 CASA系统的准确性进行评价。通过变异系数(CV)评估精子浓度、形态和渐进式活动精子(PR)百分比的精度。对精子浓度约为100 × 106 /mL的3份样品进行稀释，评价其线性范围。结果:乳珠的检出值在目标值范围内。精子浓度、PR的cv与精子浓度呈显著负相关(r = -0.561, p = 0.001)， PR值呈显著负相关(r = -0.621, p 2值在精子浓度线性范围≥0.99)。在36.5℃±0.5℃条件下，1 ~ 10 min内精子活力和PR无显著差异。GSA-810系统与手工方法分析的36份精液样本精子形态与精子头形态符合率分别为99.40%和99.67%。精子形态异常百分率和头部形态异常百分率的cv值均小于5%。结论:GSA-810系统能准确分析正常精液样本，但对少精症和弱精症样本结果重复性较差。未来用于精子形态分析的CASA系统应侧重于识别精子的中间和尾部。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Laboratory Analysis 医学-医学实验技术

CiteScore

5.60

自引率

7.40%

发文量

584

审稿时长

6-12 weeks

期刊介绍： Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.