External quality assessment scheme for sperm DNA fragmentation: a pilot study in China.

IF 2.4 3区医学 Q2 ANDROLOGY

Basic and Clinical Andrology Pub Date : 2023-11-28 DOI:10.1186/s12610-023-00211-0

Yan Zheng, Ying-Bi Wu, Ye-Lin Jia, Li-Juan Ying, Ting-Ting Yang, Qing-Yuan Cheng, Jiao Qin, Chen Luo, Lin Yu, Fu-Ping Li

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引用次数: 0

Abstract

Background: The aim of this article is to establish an external quality assessment (EQA) scheme for sperm Deoxyribonucleic acid (DNA) fragmentation (SDF) detection, and to assess the feasibility of the scheme. In addition, this article provides some case analysis of abnormal results in order to really help improve the performance of the laboratory.

Results: In 2021 and 2022, 10 and 28 laboratories in China volunteered to participate in the EQA program respectively. Two samples were selected for EQA each year, a large spread of results was obtained for the four samples, and the highest values were 13.7, 4.2, 8.0 and 4.0 times the lowest respectively. The coefficients of variation (CVs) were very high for the four samples, at 46.6%, 30.1%, 26.7% and 30.3%, respectively. The CVs of the samples with high SDF values were lower than those of the samples with low SDF values. There was no significant difference between the results of sperm chromatin structure assay (SCSA) and sperm chromatin dispersion (SCD). For the 10 laboratories that participated in EQA in 2021 and 2022, the CVs of low SDF value samples and high SDF value samples decreased from 46.6% and 30.1% in 2021 to 32.5% and 22.7% in 2022, respectively.

Conclusion: This is the first study to evaluate the EQA program on SDF, which involved a number of laboratories and was demonstrated to be feasible. It is recommended that all laboratories participate in the EQA of SDF to ensure the accuracy of the results.

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精子DNA片段化外部质量评价方案:中国试点研究。

背景:本文旨在建立精子脱氧核糖核酸(DNA)片段化(SDF)检测的外部质量评价(EQA)方案，并对该方案的可行性进行评估。此外，本文还提供了一些异常结果的案例分析，以真正帮助提高实验室的绩效。结果:在2021年和2022年，中国分别有10个和28个实验室自愿参加EQA项目。每年选取2个样本进行EQA, 4个样本的结果差异较大，最高值分别是最低值的13.7倍、4.2倍、8.0倍和4.0倍。4个样品的变异系数(cv)均非常高，分别为46.6%、30.1%、26.7%和30.3%。高SDF值样本的cv值低于低SDF值样本的cv值。精子染色质结构测定(SCSA)和精子染色质离散度测定(SCD)结果无显著差异。在2021年和2022年参与EQA的10个实验室中，低SDF值样本和高SDF值样本的cv分别从2021年的46.6%和30.1%下降到2022年的32.5%和22.7%。结论:本研究首次对SDF的EQA项目进行了评估，该项目涉及多个实验室，并被证明是可行的。建议所有实验室都参与SDF的EQA，以确保结果的准确性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Basic and Clinical Andrology Medicine-Urology

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

22 weeks

期刊介绍： Basic and Clinical Andrology is an open access journal in the domain of andrology covering all aspects of male reproductive and sexual health in both human and animal models. The journal aims to bring to light the various clinical advancements and research developments in andrology from the international community.