Enhanced periodontal tissue healing via vascular endothelial growth factor expression following low-level erbium-doped: yttrium, aluminum, and garnet laser irradiation: In vitro and in vivo studies

IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Shu Takemura, Koji Mizutani, Risako Mikami, Keita Nakagawa, Masahiro Hakariya, Eri Sakaniwa, Natsumi Saito, Hiromi Kominato, Daisuke Kido, Kohei Takeda, Akira Aoki, Takanori Iwata
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Abstract

Background

This study aimed to investigate the effects of low-level erbium-doped: yttrium, aluminum, and garnet (Er:YAG) laser irradiation on periodontal tissue healing and regeneration through angiogenesis in vivo and in vitro studies.

Methods

Intrabony defects were surgically created in the bilateral maxilla molar of rats. The defects were treated by open flap debridement (OFD) with Er:YAG laser, including low-level laser irradiation (LLLI) to bone and blood clot surfaces, or conventional procedures. The mRNA expression of vascular endothelial growth factor (VEGF) in the surgical sites was quantified using real-time polymerase chain reaction. The decalcified specimens were prepared for histometric analysis. Also, LLLI was performed on human umbilical vein endothelial cells to evaluate the effects on angiogenesis. Cell proliferation, VEGF expression, and tube formation were assessed. In addition, capsazepine (CPZ), a selective inhibitor of transient receptor potential vanilloid 1 (TRPV1), treatment was performed before LLLI for the same assays.

Results

OFD using Er:YAG laser did not generate thermal damage on bone or root surfaces. LLLI accelerated hemostasis by coagulation of the superficial layers of blood clots in the laser-treated group. Postoperative healing was sound in all animals in both groups. VEGF expression and bone formation were significantly increased in the laser-treated group compared to those in the conventional treatment group. In vitro, cell proliferation and VEGF expression were significantly increased in the LLLI group compared to the control group. Tube-formation assays showed that LLLI significantly promoted angiogenesis. CPZ treatment significantly suppressed VEGF expression and tube formation following LLLI.

Conclusions

This study suggests that Er:YAG laser irradiation may promote periodontal tissue healing by enhancing angiogenetic effect of endothelial cells via TRPV1.

低水平掺铒:钇、铝和石榴石激光照射后通过血管内皮生长因子表达增强牙周组织愈合:体外和体内研究
背景:本研究旨在探讨低水平掺铒:钇,铝和石榴石(Er:YAG)激光照射对牙周组织血管生成愈合和再生的影响。方法:对大鼠双侧上颌磨牙进行骨内缺损手术修复。缺损采用Er:YAG激光开瓣清创(OFD)治疗,包括低水平激光照射(LLLI)骨和血凝块表面,或常规手术。采用实时聚合酶链反应定量检测手术部位血管内皮生长因子(VEGF) mRNA表达。制备脱钙标本进行组织计量学分析。并对人脐静脉内皮细胞进行了LLLI实验,以评估其对血管生成的影响。评估细胞增殖、VEGF表达和管形成情况。此外,辣椒素(CPZ)是一种瞬时受体电位香草素1 (TRPV1)的选择性抑制剂,在LLLI之前进行了同样的检测。结果:Er:YAG激光OFD对骨和根表面均无热损伤。在激光治疗组中,LLLI通过凝固血凝块的浅层来加速止血。两组动物术后愈合良好。与常规治疗组相比,激光治疗组VEGF表达和骨形成明显增加。在体外,与对照组相比,LLLI组细胞增殖和VEGF表达明显增加。成管实验表明,LLLI显著促进血管生成。CPZ处理显著抑制血管内皮生长因子的表达和LLLI后的小管形成。结论:Er:YAG激光照射可能通过增强内皮细胞TRPV1血管生成作用促进牙周组织愈合。
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来源期刊
Journal of periodontology
Journal of periodontology 医学-牙科与口腔外科
CiteScore
9.10
自引率
7.00%
发文量
290
审稿时长
3-8 weeks
期刊介绍: The Journal of Periodontology publishes articles relevant to the science and practice of periodontics and related areas.
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