Differences in the range of thermoneutral zone between mouse strains: potential effects on translational research.

IF 2.2 3区 医学 Q3 PHYSIOLOGY
Andrzej K Gębczyński, Julita Sadowska, Marek Konarzewski
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引用次数: 0

Abstract

Laboratory mice are commonly used for studies emulating human metabolism. To render human energetics, their ratio of daily (DEE) to basal (BMR) energy expenditure of 1.7-1.8 should be maintained. However, the DEE/BMR ratio strongly depends on whether a given study using a mouse model is carried out above, or below the lower critical temperature (LCT) of the thermoneutral zone, which is rarely considered in translational research. Here, we used mice artificially selected for high or low rates of BMR along with literature data to analyze the effect of ambient temperature on possible systematic bias in DEE/BMR. We demonstrated that the estimated LCTs of mice from the high and low BMR lines differ by more than 7°C. Furthermore, the range of variation of LCTs of mouse strains used in translational research spans from 23 to 33°C. Differences between LCTs in our selected mice and other mouse strains are mirrored by differences in their DEE-to-BMR ratio, on average increasing it at the rate of 0.172°C-1 at temperatures below LCT. Given the wide range of LCTs in different mouse strains, we conclude that the energetic cost of thermoregulation may differ greatly for different mouse strains with a potentially large impact on translational outcomes. Thus, the LCT of a given mouse strain is an important factor that must be considered in designing translational studies.

小鼠品系间热中性区范围的差异-对转译研究的潜在影响。
实验室小鼠通常用于模拟人体代谢的研究。为了提供人体能量,他们的每日(DEE)与基础(BMR)能量消耗的比率应保持在1.7-1.8。然而,DEE/BMR比率在很大程度上取决于使用小鼠模型的给定研究是否高于或低于热中性区的较低临界温度(LCT),这在转化研究中很少考虑。在这里,我们使用人工选择的高或低BMR率的小鼠以及文献数据来分析环境温度对DEE/BMR可能的系统偏差的影响。我们证明,来自高BMR和低BMR系的小鼠的估计lct相差超过7°C。此外,用于转化研究的小鼠品系的LCTs变化范围为23至33°C。我们所选小鼠的LCT与其他小鼠品系的差异反映在它们的DEE/BMR比率的差异上,在低于LCT的温度下,其平均增加率为0.172°C-1。鉴于不同小鼠品系的lct范围广泛,我们得出结论,不同小鼠品系的体温调节的能量成本可能存在很大差异,这可能对翻译结果产生很大影响。因此,给定小鼠品系的LCT是设计转译研究时必须考虑的一个重要因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.30
自引率
3.60%
发文量
145
审稿时长
2 months
期刊介绍: The American Journal of Physiology-Regulatory, Integrative and Comparative Physiology publishes original investigations that illuminate normal or abnormal regulation and integration of physiological mechanisms at all levels of biological organization, ranging from molecules to humans, including clinical investigations. Major areas of emphasis include regulation in genetically modified animals; model organisms; development and tissue plasticity; neurohumoral control of circulation and hypertension; local control of circulation; cardiac and renal integration; thirst and volume, electrolyte homeostasis; glucose homeostasis and energy balance; appetite and obesity; inflammation and cytokines; integrative physiology of pregnancy-parturition-lactation; and thermoregulation and adaptations to exercise and environmental stress.
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