Comparison of mCP and TSC Media to Enumerate Clostridium perfringens in Surface Water Samples.

Polish journal of microbiology Pub Date : 2023-12-16 eCollection Date: 2023-12-01 DOI:10.33073/pjm-2023-039
Renata Matuszewska, Łukasz Mąka
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Abstract

The Clostridium perfringens bacteria are used to assess water quality as an indicator parameter. If detected, it can confirm the occurrence of past fecal contamination. Tests determining C. perfringens in water samples are usually performed by membrane filtration where filters are incubated on selective media under anaerobic conditions. Available media include mCP and TSC. The aim of this study was to compare the relative recovery of C. perfringens (including spores) from surface water samples and to determine the performance characteristics of the membrane filtration method using both media. The results showed that, although the procedure using the mCP medium was more sensitive and specific, higher recoveries were obtained in the tests based on the TSC medium.

mCP和TSC培养基在地表水样品中产气荚膜梭菌计数的比较。
采用产气荚膜梭菌作为评价水质的指标参数。如果检测到,它可以确认过去粪便污染的发生。测定水样中产气荚膜梭菌的试验通常通过膜过滤进行,其中过滤器在厌氧条件下在选择性培养基上孵育。可用介质包括mCP和TSC。本研究的目的是比较地表水样品中产气荚膜荚膜菌(包括孢子)的相对回收率,并确定使用两种介质的膜过滤方法的性能特征。结果表明,虽然使用mCP培养基的方法具有更高的灵敏度和特异性,但基于TSC培养基的测试获得了更高的回收率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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